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| 酶受体生物荧光法快速检测水果蔬菜中多农药残留 |
| XING Shi-Ge1, YAO Gui-hong1, YAO Mei-Yi1, LIU Yun-Fei2, LING Yun1 |
| 投稿时间：2025-04-03 修订日期：2025-05-26 |
| DOI： |
| 中文关键词: 酶受体生物荧光法 农药残留快速检测 水果蔬菜 |
| 英文关键词:Bioluminescence Inhibition of Enzyme receptor Rapid detection of multiple pesticide residues Fruits and vegetables |
| 基金项目:中国质量检验检测科学研究院 基本科研业务费项目 |
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| 中文摘要: |
| 目的 经过对酶受体生物荧光法进行条件优化，确定最佳前处理方法及检测条件，建立水果和蔬菜中农药多残留检测快速检测方法。结果 该方法可对水果蔬菜中的多种农药残留同时检测，灵敏度满足国家标准GB 2763的限量要求，假阴性率为0%，假阳性率<5%，交叉反应率≤0.01%。采用酶受体生物荧光法和国标方法对市售水果和蔬菜样品进行检测，结果一致。结论 本研究建立的酶受体生物荧光法可用于水果和蔬菜中农药残留快速测定。 |
| 英文摘要: |
| Objective To investigate a rapid analytical protocol for the detection of multiple pesticide residues in fruits and vegetables on Bioluminescence Inhibition of Enzyme receptor. Methods Within the context of this investigation, the enzyme receptor bioluminescence technique was employed, and its pretreatment protocol and detection conditions parameters were optimized. Subsequently, the analyte-spiked samples underwent comprehensive analysis. Results The findings demonstrated that the enzyme receptor bioluminescence technique is capable of concurrently identifying several pesticide residues within fruits and vegetables, with a sensitivity that complies with the national standard GB 2763. The false negative rate =0%, the false positive rate＜5%, and the cross-reactivity rate≤0.01%. Comparative analysis utilizing both the enzyme receptor bioluminescence technique and the conventional national standard method was conducted on fruit and vegetable samples sourced from the market, yielding congruent results. Conclusion The enzyme receptor bioluminescence method established in the present study is deemed suitable for the rapid assessment of pesticide residues in fruits and vegetables. |
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