| Objective This study focuses on evaluating the safety of a composite beverage made from white reishi mushroom-fermented Pu'er tea, laying a scientific foundation for its widespread application in the food industry and related fields. Methods Using the white reishi mushroom fermented Pu'er tea composite beverage as the study subject, based on preliminary experiments and related research, the dose was set at 10,000 mg/kg bw. The classical limit method was employed, with healthy ICR mice selected as the experimental model, and the test substance was administered via oral gavage. The experiment strictly adhered to animal ethics and welfare principles, with precise control of environmental conditions including temperature, humidity, and light cycles to ensure the accuracy and reliability of the data. Results After administration of the test substance, no signs of toxicity were observed in any of the ICR mice throughout the observation period. Their behavioral activity remained normal, with glossy fur and vigorous spirits. Regular precise measurements of mouse body weight showed no significant abnormalities in growth trends, and no deaths occurred. Statistical analysis revealed that the acute oral toxicity LD50 values for both male and female ICR mice were greater than 10,000 mg/kg bw, with 95% confidence intervals also exceeding 10,000 mg/kg bw. Conclusion Based on international acute toxicity dose classification standards, the white reishi mushroom fermented Pu'er tea composite beverage is classified as practically non-toxic. This indicates that under the experimental conditions of this study, the acute oral intake safety of this composite beverage is high, providing critical safety data support for subsequent product development and market promotion. |
