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| 黑曲霉改性梨渣膳食纤维的工艺优化及其生理活性和保健功能研究 |
| Process optimization of altering the properties of pear residue soluble dietary fiber by Aspergillus niger and study on its physiological activity and health function |
| 投稿时间：2025-03-12 修订日期：2025-05-14 |
| DOI： |
| 中文关键词: 梨渣膳食纤维 黑曲霉 工艺优化 生理活性 保健功能 |
| 英文关键词:Pear residue soluble dietary fiber Aspergillus niger Process optimization Physiological activity Health function |
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| 中文摘要: |
| 摘 要：目的 优化黑曲霉改性梨渣膳食纤维(pear residue soluble dietary fiber, PSDF)发酵工艺，并对其特性进行研究。方法 利用单因素实验，优化黑曲霉的接种量、发酵温度和发酵时间，确定最佳发酵工艺；通过测定PSDF的持水性(water holding capacity，WHC)，持油性(oil holding capacity，OBC)，果糖、葡萄糖、阿拉伯糖、木糖和鼠李糖含量，1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl radical，DPPH)自由基清除能力，2,2’-联氮-双-3-乙基苯并噻唑啉-6-磺酸[2,2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate)，ABTS]阳离子自由基清除能力，葡萄糖吸附能力(glucose adsorption capacity，GAC)，胆固醇吸附能力(cholesterol adsorption capacity，CAC)，确定改性后PSDF的特性。结果 最佳发酵工艺为：黑曲霉的接种量为8%，发酵温度为36 ℃，发酵时间为7 d；PSDF的WHC为15.89 g/g，OBC为4.02 g/g；果糖、葡萄糖、阿拉伯糖的含量提高，木糖和鼠李糖的含量降低；DPPH自由基清除能力和ABTS+自由基清除能力均显著提高；GAC提高至0.77 mg/g，CAC提高至0.56 mg/g。结论 适宜的发酵工艺改性PSDF，有助于提高得率。黑曲霉改性PSDF有效地改善了其生理活性和保健功能。 |
| 英文摘要: |
| ABSTRACT: Objective To optimize the fermentation process of pear residue soluble dietary fiber (PSDF) modified by Aspergillus niger and study its characteristics. Methods Using single factor experiments to optimize the inoculation amount, fermentation temperature, and fermentation time of Aspergillus niger, and determine the optimal fermentation process; determine the characteristics of the modified PSDF: The WHC, OBC, fructose, glucose, arabinose, xylose and xylose content, 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) free radical scavenging ability, 2,2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) cationic radical scavenging ability, GAC and CAC. Results By optimizing the fermentation process, the optimal inoculation amount, fermentation temperature, and fermentation time of Aspergillus niger were determined to be 8%, 36 ℃, and 7 d. Based on the optimal fermentation process, the characteristic analysis of PSDF was conducted: WHC was 15.89 g/g, and OBC was 4.02 g/g; the content of fructose, glucose, and arabinose increases, while the content of xylose and xylose decreases; the DPPH radical scavenging ability and ABTS+ radical scavenging ability were significantly improved; GAC increased to 0.77 mg/g, and CAC increased to 0.56 mg/g. Conclusion Modifying PSDF with suitable fermentation processes can help improve yield. The use of Aspergillus niger modified pear pomace dietary fiber effectively improved its physiological activity and health function. |
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