|
| 二氧化铈/多壁碳纳米管/乙炔黑纳米电化学传感器法快速测定苦荞总黄酮 |
| Rapid Determination of Total Flavonoids in Tartary Buckwheat by CeO2/MWCNTs/Acetylene Black Nano-electrochemical Sensor method |
| 投稿时间：2025-02-23 修订日期：2025-06-10 |
| DOI： |
| 中文关键词: 荞麦 总黄酮 纳米材料 电化学传感器 |
| 英文关键词:Buckwheat Total Flavonoids Nanomaterials Electrochemical sensor |
| 基金项目:河北省重点研发计划项目(21327111D) |
|
|
|
|
| 摘要点击次数: 7 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的 黄酮类化合物是苦荞麦中最主要的生物活性物质,具有抗氧化、改善血管微循环、调节糖脂代谢、降尿酸等生理调节功效。本文以二氧化铈(CeO2)、多壁碳纳米管(MWCNTs)、乙炔黑为复合修饰材料固定化于玻碳电极(GCE)表面,制备了CeO2/MWCNTs/乙炔黑/GCE电化学传感器,建立了一种快速检测食品中黄酮类化合物的方法。方法 采用扫描电镜、循环伏安法、阻抗技术对修饰材料及其传感器进行了表征,其循环伏安图表明在0.54 V、0.57 V出现一对峰形良好的氧化峰和还原峰,说明黄酮类化合物电化学氧化还原反应是可逆的。结果 对试验条件进行了优化,结果如下：以pH 为2.0的B-R溶液作为电解质溶液,复合修饰材料用量12 uL,CeO2/MWCNTs与乙炔黑的比例1:3,富集时间为9 min,苦荞黄酮氧化峰电流与其浓度之间呈良好的线性关系,线性方程为Ip=3.508+0.793C(R2=0.99745),最低检测限可达到6.5×10-8 mol/L。该方法用于苦荞麦中总黄酮的检测,其加标回收率为99.46%~102.15%。结论 该方法的检测结果优于紫外-可见光分光光度法,同时具有样品预处理简单、快捷方便、检测成本低、灵敏度高、准确性好等优势,可用于食品中总黄酮快速准确的分析。 |
| 英文摘要: |
| ABSTRACT: Objective To establish a rapid method for determining total flavonoids in tartary buckwheat using a nano-electrochemical sensor based on cerium dioxide/multi-walled carbon nanotubes/acetylene black (CeO2/MWCNTs/AB). Methods The modified material and these nanoelectrochemical sensors were characterized by scanning electron microscopy, cyclic voltammetry and Electrochemical impedance spectroscopy.The electrochemical behavior of rutin on the electrode surface was systematically investigated via CV.Critical parameters were optimized, including electrolyte type , pH, modifier ratio , coating volume , accumulation time and scan rate. Results One pair of well- defined redox peaks were observed at the potential of 0.54 V and 0.57 V, which indicated that the electrochemical oxidation and reduction reaction of flavonoid is reversible.The experimental conditions were optimized, the results as follows: B-R solution with pH 2.0 as the electrolyte solution, amounts of modified material, 12 μL, VCeO2/MWCNTs:Vacetylene black as 1:3, enrichment time as 9 min. At these conditions, the oxidation peak current of flavonoid linearly increased with its concentration, Ip=3.508+0.793C (r2=0.99745), and the detection limit was 6.5×10-8 mol/L. The developed method was used to determinate total flavonoids content of Tartary buckwheat, the recoveries are in the range of 99.46%-102.15%, the results were superior to Ultraviolet-visible spectrophotometry. Conclusion The method is feasible for the rapid and accurate determination of total flavonoids in food with the advantages such as simple pretreatment time saving and convenience, low detection cost, high sensitivity and good accuracy. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
