| ABSTRACT: Objective A fully automated method for the simultaneous determination of 11 synthetic colorants in beverages by solid phase extraction and chromatography was established. Methods Take 2 g of beverage sample, extract it with ammonia ethanol solution, and perform operations such as activation, sample loading, washing eluting, and collection in turn with the help of a fully automatic solid phase extraction instrument. After nitrogen blowing and redissolution, the liquid chromatography instrument [diode multi-wavelength multi-channel simultaneous detection, detection wavelength 415 nm (Citrus Yellow, Quinoline Yellow), 520 nm (New Red Amaranth, Carmine, Sunset Yellow, Temptation Red, Acidic Red, and Red Algae), 610 nm (Indigo, Bright Blue mobile phase: A 20 mmol/L ammonium acetate solution, B methanol, flow rate 1 ml/min gradient elution] was used on-line determination. Results In the range of 0.2~10.0 μg/mL, the concentration of 11 synthetic colorants showed good linear relationship with peak area, and the linear correlation coefficients were all greater than 0.99, the limit of detection met the requirements of GB 500935—2023 "National Food Safety Standard for the Determination of Synthetic Colorants in Food". In the standard addition method recovery test, the recovery rate between 92.5% and 106%, and the relative standard deviation of the determination value (n=6) was less than 5.0% This method was applied to the ability verification of Sunset Yellow and Carmine in beverages in food testing institutions in the Yangtze River Delta. The content of Carmine was 586 mg/kg, and the content of Sunset Yellow was 4.31 mg/kg. The other synthetic colorants were not detected. Conclusions This method achieve good enrichment effect, high automation degree, simple operation, good stability, short time, high efficiency, good sensitivity, high accuracy, and good reproducibility, and is suitable the determination and analysis of synthetic colorants in a large number of beverages. |
