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| 土壤杆菌A02分批发酵产可得然胶动力学模型的建立 |
| Establishment of a batch fermentation kinetic model for the production of curdlan by Agrobacterium sp.A02 |
| 投稿时间：2025-02-10 修订日期：2025-04-08 |
| DOI： |
| 中文关键词: 土壤杆菌 可得然胶 发酵动力学模型 分批发酵 |
| 英文关键词:Agrobacterium sp. curdlan fermentation kinetic model batch fermentation |
| 基金项目:宁夏自然科学(2023AAC03735)；2023年度宁夏回族自治区青年科技托举人才培养项目(宁科协发组字〔2024〕6号)；宁夏科技惠民项目(2024CMG03049) |
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| 中文摘要: |
| 目的 作为新型食品添加剂可得然胶的主要生产菌种,土壤杆菌的产胶能力备受关注。为预测和控制土壤杆菌A02发酵产可得然胶的过程,建立随时间变化的模型,模拟细菌的生长,可得然胶生成以及蔗糖消耗的动力学变化。方法 对土壤杆菌分批发酵过程中的菌体浓度、蔗糖含量、可得然胶产量进行测定,分别采用Logistic方程、Luedeking- Piret 方程和Luedeking- Piret -Like方程对实验值进行拟合。结果:拟合结果表明构建的3种模型适用于描述菌体生长动力学、可得然胶生成动力学和蔗糖消耗动力学,其R2值均在0.99以上,显著性水平均为极显著。模型拟合值与实验值误差均小于10%,拟合良好。结论 建立的3个动力学模型都能较好的描述和预测土壤杆菌A02在发酵产可得然胶过程中的动态代谢变化。 |
| 英文摘要: |
| Objective As the main production strain of the new food additive, Agrobacterium sp.has attracted much attention for its ability to curdlan. To predict and control the fermentation process of Agrobacterium sp. A02 strain to produce curdlan, a time-varying model was established to simulate bacterial growth and obtain the kinetic changes of curdlan production and sucrose consumption.? Methods The viable bacteria count, curdlan content, and sucrose content during the batch fermentation process of Agrobacterium sp. A02 were measured, and the experimental values were fitted using logistic equation, Luedeking-Piret equation, and Luedeking-Piret-Like equation, respectively. Results The fitting results indicate that the three models are applicable to the growth kinetics of bacterial cells, the generation kinetics of pectin, and the consumption kinetics of sucrose. The R2 values were above 0.99, and the significance level is extremely significant.The errors between the fitted and experimental values were less than 10%, indicating a good fitand.Conclusion This suggests that the three kinetic models established can predict and describe the dynamic metabolic changes of Agrobacterium sp. A02 during the fermentation process. |
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