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| 基于网络药理学和动物实验探究海参肠卵肽增强小鼠免疫功能的作用及机制 |
| Effects and mechanisms of sea cucumber intestinal ovigerm peptides on immune function in mice through network pharmacology and animal experiments |
| 投稿时间：2025-01-28 修订日期：2025-05-16 |
| DOI： |
| 中文关键词: 海参肠卵肽 网络药理学 有效成分 靶点 免疫增强 作用机制 |
| 英文关键词:Sea cucumber intestinal ovigerm peptides Network pharmacology Effective components Targets Immune enhancement Mechanism of action |
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| 中文摘要: |
| 摘 要: 目的 通过网络药理学和动物实验探讨海参肠卵肽(sea cucumber intestinal ovigerm peptides,SCIOP)增强小鼠免疫功能的作用与机制。方法 通过数据库筛选SCIOP的活性成分和免疫细胞的靶点，并对核心靶点进行基因本体(gene ontology, GO)富集分析以及京都基因和基因组百科全书(kyoto encyclopedia of genes and genomes, KEGG)富集分析，初步挖掘SCIOP增强免疫功能的可能机制。动物实验选取32只4-5周龄雄性昆明小鼠(kunming mice, KM)，随机分为4组，实验组分别以0.52 g/kg(SCIOP-L组)和1.04 g/kg(SCIOP-H组)的SCIOP灌胃，阳性对照组灌胃0.52 g/kg海参壁肽，空白对照灌胃等量蒸馏水。30天灌胃处理后，测定淋巴器官/体重比、迟发型变态反应、脾淋巴细胞转化能力、体液免疫和腹腔巨噬细胞吞噬活性等各项免疫指标。结果 网络药理学结果显示SCIOP增强免疫功能的活性成分可能为黄瓜苷、海参素等15种化学成分，关键靶点可能为细胞分裂周期25A(cell division cycle 25A, CDC25A)、成纤维细胞生长因子1(fibroblast growth factor 1, FGF1)等46个免疫靶点，富集在细胞黏附分子、缺氧诱导因子-1(hypoxia-inducible factor-1,HIF-1)等14条信号通路。动物实验结果表明SCIOP能显著增加小鼠迟发型变态反应、抗体生成水平、腹腔巨噬细胞吞噬能力和脾淋巴细胞转化能力。结论 SCIOP激活免疫细胞具有多成分、多靶点、多通路的特点，可能通过多种活性成分作用于多核心靶点，调控多条信号通路来发挥增强免疫功能的作用。 |
| 英文摘要: |
| ABSTRACT：Objective To explore the effects and mechanisms of sea cucumber intestinal ovigerm peptides(SCIOP) on enhancing the immune function of mice through network pharmacology and animal experiments. Methods The database was used to screen the active chemical components of SCIOP and the targets of immune cells.Gene Ontology (GO) functional analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were conducted on the relevant core targets to preliminarily explore the potential targets and mechanisms of SCIOP in enhancing immune function. In the animal experiments, 32 male kunming mice (4-5 weeks old) were randomly assigned to 4 groups. The experimental groups were treated with SCIOP at doses of 0.52 g/kg (SCIOP-L) and 1.04 g/kg (SCIOP-H) via gavage, the positive control group received 0.52 g/kg of? sea cucumber peptides, while the blank control group was given an equal volume of distilled water by gavage. After 30 days of gavage, immune indicators such as lymphoid organ/body weight ratio, delayed-type hypersensitivity, splenic lymphocyte transformation capacity, humoral immunity, and peritoneal macrophage phagocytic activity were measured. Results The results of network pharmacology showed that the active ingredients of SCIOP in enhancing immune function may be cucumarioside, holothurin and other fifteen ingredients. The key targets may be cell division cycle 25A(CDC25A), fibroblast growth factor 1(FGF1) and other forty-six immune targets, which mainly enriched in cell adhesion molecules, hypoxia-inducible factor-1 and other fourteen signaling pathways. Animal experiment results showed that SCIOP significantly increased delayed-type hypersensitivity, antibody production levels, the phagocytic capacity of peritoneal macrophages, and splenic lymphocyte transformation capacity. Conclusion SCIOP activate immune cells with characteristics of multiple components, multiple targets, and multiple pathways, which may be exert their immune-enhancing effects through?various?active components acting on multiple core targets and regulating a number of signaling pathways. |
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