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| 酶解超声联合提取桑椹多糖及其抗氧化能力和降血糖活性研究 |
| Study on the enzymatic hydrolysis combined with ultrasonication extraction of Mori Fructus Polysaccharide: Antioxidant and Hypoglycemic Activities |
| 投稿时间：2025-01-15 修订日期：2025-04-17 |
| DOI： |
| 中文关键词: 桑椹多糖 酶解超声联合提取法 抗氧化能力 α-淀粉酶活性 α-葡萄糖苷酶活性 |
| 英文关键词:Mori Fructus polysaccharides enzymatic hydrolysis combined with ultrasound extraction method antioxidant power α-amylase activity α-glucosidase activity |
| 基金项目:国家自然科学基金项目（面上项目，重点项目，重大项目）；、浙江省“三农九方”科技协作项目 |
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| Author | Institution |
| ZHAO Xin-Lei | School of Food Science and Engineering,Shaanxi University of Science and Technology |
| LIU Ning | School of Food Science and Engineering,Shaanxi University of Science and Technology |
| XIA Qi-Le | Key Laboratory of Post-harvest Handling of Fruits,Ministry of Agriculture and RuralAffairs,Key Laboratory of Post-harvest Preservation and Processing of Vegetables Co-construction by Ministry and Province,Ministry of Agriculture and Rural Affairs,Provincial Key Laboratory of Intelligent Logistics and Processing of Fresh Food,Institute of Food Science,Zhejiang Academy of Agricultural Sciences |
| LIU Chen-Xing | Key Laboratory of Post-harvest Handling of Fruits,Ministry of Agriculture and RuralAffairs,Key Laboratory of Post-harvest Preservation and Processing of Vegetables Co-construction by Ministry and Province,Ministry of Agriculture and Rural Affairs,Provincial Key Laboratory of Intelligent Logistics and Processing of Fresh Food,Institute of Food Science,Zhejiang Academy of Agricultural Sciences |
| CAO Yan | Key Laboratory of Post-harvest Handling of Fruits,Ministry of Agriculture and RuralAffairs,Key Laboratory of Post-harvest Preservation and Processing of Vegetables Co-construction by Ministry and Province,Ministry of Agriculture and Rural Affairs,Provincial Key Laboratory of Intelligent Logistics and Processing of Fresh Food,Institute of Food Science,Zhejiang Academy of Agricultural Sciences |
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| 中文摘要: |
| 目的 研究酶解超声联合提取条件对桑椹多糖得率及其抗氧化能力和降血糖活性的影响。方法 以桑椹果渣为原料，以桑椹多糖得率为指标，通过单因素试验优化提取工艺，并对热水浸提所得多糖（hot water extracting polysaccharide, HWP）、酶解辅助提取所得多糖（enzyme-assisted extracting polysaccharide, EWP）、超声辅助提取所得多糖（ultrasound-assisted extracting polysaccharide, UWP）、酶解超声联合提取所得多糖（enzymatic hydrolysis combined with ultrasonication extracting polysaccharide, EUP）的DPPH自由基清除率、ABTS+自由基清除率、α-淀粉酶和α-葡萄糖苷酶活性抑制率进行研究。结果 酶解超声联合提取桑椹多糖的最佳条件为料液比1:80 （g/mL）、添加2%复合酶（纤维素酶和果胶酶体积比为1:1）、50.0 ℃酶解2 h后，于400 W、60.0 ℃超声提取50.0 min，该条件下EUP得率为4.81%，分别比HWP、EWP、UWP提高了7.6%、17.5%、21.6%。EUP对ABTS+自由基和DPPH自由基的清除率也有所提高。EUP对α-淀粉酶活性的半抑制浓度（semi-inhibitory concentration, IC50）为1.18 mg/mL，比HWP、EWP和UWP分别降低了40.1%、18.6%、30.6%；对α-葡萄糖苷酶活性的IC50为0.31 mg/mL，比HWP、EWP和UWP分别降低了16.8%、8.0%、39.4%。结论 采用酶解超声联合提取法既能提高桑椹多糖得率，还可提高其抗氧化能力和降血糖活性，具有较好的应用前景。 |
| 英文摘要: |
| Objective To investigate the effects of enzymatic hydrolysis combined with ultrasonication extraction conditions on the yield, antioxidant and hypoglycemic activities of Mori Fructus polysaccharides, this study optimized extraction parameters and evaluated the functional properties. Methods Using Mori Fructus pomace as the raw material and the yield of Mori Fructus polysaccharide as the index, the extraction conditions was optimized through single-factor experiments. Then the DPPH radical scavenging capacity, ABTS+ radical scavenging capacity, and the inhibitory effects on α-amylase and α-glucosidase activity of hot water extracting polysaccharide (HWP), enzyme-assisted extracting polysaccharide (EWP), ultrasound-assisted extracting polysaccharide (UWP), enzymatic hydrolysis combined with ultrasonication extracting polysaccharide (EUP) were investigated. Results The optimal conditions for polysaccharide extraction by enzymatic hydrolysis combined with ultrasonication was obtained. Mori Fructus pomace powder was mixed with water at ratio of 1:80 (g/mL), and 2% composite enzyme (cellulase and pectinase at a ratio of 1:1, V:V) was added in the mixture which was maintained at 50.0 ℃ for 2 h with stirring. Then the mixture was treated by ultrasonication at 400.0 W and 60.0 ℃ for 50.0 min, and the final yield of EUP was 4.81%, increased by 7.6%, 17.5% and 21.6% compared with HWP, EWP and UWP. EUP also exhibited an improvement in the scavenging activity of both ABTS+ radical and DPPH radical. Compared with HWP, EWP and UWP, the semi-inhibitory concentration (IC50) of α-amylase activity of EUP was 1.18 mg/mL, reduced by 40.1%, 18.6% and 30.6%, respectively, and the IC50 of α-glucosidase activity of EUP was 0.31 mg/mL, reduced by 16.8%, 8.0% and 39.4%, respectively. Conclusion The enzymatic hydrolysis combined with ultrasonication extraction can not only improve the Mori Fructus polysaccharide yield, but also enhance the antioxidant power and hypoglycemic activity of Mori Fructus polysaccharide, which has a potential application prospect in the industry. |
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