| Objective To investigate the efficacy of throat lozenge concentrate in promoting the resolution of bacterial inflammation. Methods Danio rerio model of lipopolysaccharide-induced bacterial inflammation was employed. In order to analyze the efficacy in bacterial inflammation resolution, the transgenic Danio rerio at 3-day post-fertilization was randomly selected for setting a normal control group, a model experimental group, a positive control group, and throat lozenge concentrate (at the mass concentrations of 62.5, 125.0, and 250.0 μg/mL) dosage groups. Results Throat lozenge concentrate was found to reduce the amount of neutrophils, decrease the fluorescence intensity of macrophages, lower the NO fluorescence intensity at the inflammatory site, and downregulate the relative expression levels of inos, cox2, myd88, erk2, nf-κb, il-1β, and tnf-α genes, while upregulating the relative expression level of the iκb-α gene. However, the downregulation of the relative expression level of the il-6 gene was not obvious. Conclusion Throat lozenge concentrate exhibited a certain efficacy in promoting bacterial inflammation resolution. |
