云南不同来源齿瓣石斛中氨基酸营养价值评价
Evaluation on the nutritional value of amino acids in Dendrobium devonianum Paxt.from different sources
投稿时间:2024-11-20  修订日期:2025-02-17
DOI:
中文关键词:  齿瓣石斛  氨基酸  主成分分析  聚类分析
英文关键词:Dendrobium devonianum Paxt.  amino acid  principal component analysis  clustering analysis
基金项目:英才兴边计划德宏州技术创新人才项目(No. 2023RC005);科技人才与平台计划(No. 202205AD160041)
作者单位
乔禹 云南省德宏热带农业科学研究所 
李庆聪 云南省德宏热带农业科学研究所 
冯岩晃 德宏州林业科学研究所 
李泽生 云南省德宏热带农业科学研究所 
胡永亮 云南省德宏热带农业科学研究所 
陈玉芹 云南省德宏热带农业科学研究所 
蔡文良 德宏州林业科学研究所 
AuthorInstitution
QIAO Yu Dehong Tropical Agriculture Institute of Yunnan 
LI Qing-Cong Dehong Tropical Agriculture Institute of Yunnan 
FENG Yan-Huang Dehong Research Institute of Forestry 
Li ze-sheng Dehong Tropical Agriculture Institute of Yunnan 
HU Yong-Liang Dehong Tropical Agriculture Institute of Yunnan 
chenyuqin Dehong Tropical Agriculture Institute of Yunnan 
CAI Wen-Liang Dehong Research Institute of Forestry 
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中文摘要:
      目的 评价不同来源齿瓣石斛氨基酸含量的营养价值。方法 采用氨基酸分析仪法测定齿瓣石斛的氨基酸含量,通过多元统计分析方法评价其氨基酸营养价值。结果 29个石斛样品总氨基酸含量在42.00~125.40 mg/g之间;必需氨基酸含量在16.80~44.60 mg/g之间;天冬氨酸、谷氨酸、亮氨酸和精氨酸含量最为丰富,分别为11.18 mg/g、9.97 mg/g、6.91 mg/g和5.52 mg/g,可作为齿瓣石斛的特征性氨基酸,胱氨酸(0.92 mg/g)和蛋氨酸(1.10 mg/g)分别是第一和第二限制性氨基酸;根据联合国粮食及农业组织和世界卫生组织(Food and Agriculture Organization of the United Nations/World Health Organization,FAO/WHO)氨基酸模式谱标准,梁河地区大棚基质栽培的齿瓣石斛(EAA/NEAA为62.69~77.21%;EAA/TAA为38.63~43.55%)和野生品种(EAA/NEAA为61.59~65.43%;EAA/TAA为38.12~39.56%)的营养价值最高,更符合人体需求;根据品质评价模型的综合得分排名,来源于芒市M10、M1、M7和龙陵L4、L5的样品价值较高。通过主成分分析提取到的两个主成分,累计贡献度为83.852%,建立了齿瓣石斛品质评价模型。以氨基酸和综合得分为变量进行聚类分析,可将所有样品分为3大类。结论 齿瓣石斛的氨基酸营养丰富,且不同来源的齿瓣石斛氨基酸含量有差异。该研究为齿瓣石斛的营养价值开发和利用提供了理论依据,同时,建议关注石斛的来源与栽培方式以提升市场价值。
英文摘要:
      Objective To evaluate the nutritional value of amino acid contents in Dendrobium devonianum Paxt. from different sources. Methods The amino acid analyzer method was used to determine the amino acid content of Dendrobium devonianum Paxt., and the amino acid nutritional value was evaluated by multivariate statistical analysis methods. Results The total amino acid content in 29 samples ranged from 42.00 to 125.40 mg/g; the essential amino acid content ranged from 16.80 to 44.60 mg/g; the contents of aspartic acid, glutamic acid, leucine and arginine were the most abundant, which were 11.18 mg/g, 9.97 mg/g, 6.91 mg/g and 5.52 mg/g respectively, and could be used as the characteristic amino acids of Dendrobium devonianum Paxt.. The contents of cystine (0.92 mg/g) and methionine (1.10 mg/g) were the first and second limiting amino acids respectively; according to the amino acid pattern spectrum standards of the Food and Agriculture Organization of the United Nations/World Health Organization (FAO/WHO), the nutritional value of Dendrobium devonianum Paxt. cultivated in greenhouse substrates in Lianghe area (Essential amino acid/Non-essential amino acid, EAA/NEAA was 62.69-77.21%; EAA/TAA was 38.63-43.55%) and wild varieties (EAA/NEAA was 61.59-65.43%; EAA/TAA was 38.12-39.56%) was the highest and more in line with human needs; According to the comprehensive score ranking in the quality evaluation model, the value of the samples from Mangshi M10, M1, M7 and Longling L4, L5 were higher. Two principal components were extracted by principal component analysis, and the cumulative contribution degree was 83.852%, and a quality evaluation model of Dendrobium devonianum Paxt. was established. Cluster analysis was carried out with amino acids and comprehensive scores as variables, and all samples could be divided into three major categories. Conclusion The amino acids of Dendrobium devonianum Paxt. is rich in nutrients, and the amino acid content of Dendrobium devonianum Paxt. from different sources varies. This study provided an important theoretical basis for the development and utilization of the nutritional value of Dendrobium devonianum Paxt.. At the same time, it was recommended to pay attention to the origin and cultivation methods of Dendrobium to enhance its market value.
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