酶受体生物荧光法快速检测水果和蔬菜中多农药残留

Rapid determination of multiple pesticide residues in fruits and vegetables on bioluminescence inhibition of enzyme receptor

中文关键词:  酶受体生物荧光法  农药残留快速检测  水果蔬菜

英文关键词:bioluminescence inhibition of enzyme receptor  pesticide residues  rapid detection  fruits  vegetables

中文摘要:

      目的 建立酶受体生物荧光法快速检测水果和蔬菜中多农药残留的方法。方法 以灵敏度为指标, 分别考察提取溶剂、反应时间对结果的影响, 确定最佳前处理方法及检测条件; 对方法的灵敏度、假阳性率、假阴性率和交叉反应性进行评价。结果 该方法同时检测水果蔬菜中的多种农药残留, 灵敏度满足GB 2763—2021《食品安全国家标准 食品中农药最大残留限量》的限量要求, 假阴性率为0%, 假阳性率<5%, 交叉反应率≤0.10%。采用酶受体生物荧光法和国家标准方法对市售水果和蔬菜样品进行检测, 结果一致。结论 本研究建立的酶受体生物荧光法可用于水果和蔬菜中农药残留快速测定, 适用于基层监管部门进行监管, 也有助于企业内部更好地进行质量控制, 对于提升农药残留的快速检测能力具有重要意义。

英文摘要:

      Objective To establish a rapid analytical protocol for the detection of multiple pesticide residues in fruits and vegetables on bioluminescence inhibition of enzyme receptor. Methods Regarding sensitivity as the index of investigating, the effects of the extraction solvent and reaction time were separately investigated. The pretreatment protocol and detection conditions parameters were optimized. Then, the sensitivity, false positive rate, false negative rate and cross-reactivity of the method were evaluated. Results The findings demonstrated that the enzyme receptor bioluminescence technique was capable of concurrently identifying several pesticide residues within fruits and vegetables, with a sensitivity that complies with the GB 2763—2021 National food safety standards-Maximum residue limits of pesticides in food. The false negative rate was 0%, the false positive rate was less than 5%, and the cross-reactivity rate was less than or equal to 0.10%. Comparative analysis utilizing both the bioluminescence inhibition of enzyme receptor and the conventional national standard method was conducted on fruit and vegetable samples sourced from the market, yielding congruent results. Conclusion The bioluminescence inhibition of enzyme receptor established in the present study is deemed suitable for the rapid assessment of pesticide residues in fruits and vegetables. It is appropriated for oversight by local regulatory bodies and assists companies in improving their internal quality control, which is highly important for boosting the quick detection ability of pesticide residues.