分子荧光差异加标法快速测定乳品中的噻菌灵残留量

Rapid determination of thiabendazole residue in dairy products by molecular fluorescence differential addition method

中文关键词:  噻菌灵  分子荧光  差异加标法  乳品

英文关键词:thiabendazole  molecular fluorescence  differential standard addition method  dairy products

中文摘要:

      目的 建立分子荧光差异加标法快速检测乳品中的噻菌灵残留的方法。方法 通过单因素试验确定分子荧光差异加标法的最佳试验条件并将其应用与乳品中噻菌灵的测定。结果 乳品中噻菌灵残留含量为0.1580~0.1611 mg/kg, 检出限为0.0018 μg/mL, 定量限为0.0060 μg/mL, 测定结果的相对标准偏差为0.75% (n=6), 加标回收率为99.4%~106.7%。与GB 23200.87—2016《食品安全国家标准 乳及乳制品中噻菌灵残留量的测定 荧光分光光度法》作对比, 经过F检验和t检验分析, 两者之间不存在显著性差异。结论 该方法无需绘制工作曲线和测定空白溶液, 具有操作简便、灵敏、快速、加标回收率高和结果准确等优点, 适用于乳品中噻菌灵残留的快速检测, 为测定乳品中噻菌灵残留提供了一种新的检测技术。

英文摘要:

      Objective To explore a method for rapid detection of thiabendazole residue in dairy products using molecular fluorescence differential addition method. Methods The optimal experimental conditions for the molecular fluorescence differential addition method were determined through single factor experiments, and this method was applied to determine the content of thiabendazole in dairy products. Results The residue of thiabendazole in dairy products ranged from 0.1580 to 0.1611 mg/kg, with a limit of detection was 0.0018 μg/mL and a limit of quantification was 0.0060 μg/mL. The relative standard deviation of the results was 0.75% (n=6), and the recovery rates ranged from 99.4% to 106.7%. A comparison with GB 23200.87—2016 National standards for food safety-Determination of thiabendazole residues in milk and dairy products-Fluorescence spectrophotometry showed no significant differences between the two methods based on F-test and t-test analysis. Conclusion This method eliminates the need to construct a calibration curve and measure blank solutions, offering advantages such as simplicity, sensitivity, rapidity, high recovery rates and accurate results. The proposed method is suitable for the rapid detection of thiabendazole residue in dairy products and provides a new detection technique for the determination of thiabendazole residues in dairy products.