基于差分脉冲伏安法的奶粉中三聚氰胺检测研究

Determination of Melamine in Milk Powder by Differential Pulse Voltammetry

中文关键词:  差分脉冲伏安法  多壁碳纳米管  玻碳电极  三聚氰胺  奶粉

英文关键词:Differential pulse Voltammetry  Multi-walled carbon nanotube  Glassy carbon electrode  Melamine  Milk power

中文摘要:

      摘 要：建立一种基于多壁碳纳米管（Multi-Walled Carbon NanoTube，MWNT）修饰电极的新型电化学传感平台，用于乳制品中三聚氰胺（Melamine，MEL）的快速检测，为设计便携式食品安全监测设备奠定基础。方法 本研究采用电化学循环伏安法（Cyclic Voltammetry, CV）和差分脉冲伏安法(Differential Pulse Voltammetry，DPV)，利用多壁碳纳米管)修饰的玻碳电极(Glassy Carbon Electrode，GCE)做探针，成功构建三聚氰胺检测的电化学传感器，并对实际样品奶粉中的三聚氰胺进行检测与分析。 结果 裸GCE经MWNT修饰后有效面积可以提高1.3倍， MWNT修饰电极对体系电化学反应有促进作用，MWNT修饰电极做探针的MEL电化学传感器的线性范围是10~40 μmol/L、灵敏度为23.85 μA·mmol-1·cm-2，检测到实际奶粉样品中MEL的含量符合国标要求。 结论 利用MWNT修饰电极构建MEL电化学传感器是可行的，该MEL传感器构建方法简单、重复性和稳定性良好，具有一定的抗干扰能力，准确度优异，建议推广使用。

英文摘要:

      ABSTRACT：Objective This study proposes a novel electrochemical sensing platform based on the electrode modified with multi-walled carbon nanotube (MWCNT) for the rapid detection of melamine (MEL) in milk powder, which lays a foundation for designing portable devices to enhance food safety surveillance. Methods An electrochemical sensor for melamine detection is developed by employing cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques, using a MWCNT-modified glassy carbon electrode (GCE) as the sensing probe. The constructed sensor is successfully applied to detect and analyze melamine in real-world milk powder samples. Results After modification with MWCNT, the effective surface area of the bare GCE increased by 1.3-fold. The MWCNT-modified electrode significantly enhanced the electrochemical reaction kinetics of the system. The MEL electrochemical sensor, using the MWCNT-modified GCE as the probe, exhibited a linear detection range of 10–40 μmol/L and a sensitivity of 23.85 μA·mM?1·cm?2. The detected MEL content in real milk powder samples complied with the Chinese National Standard (GB) requirements. Conclusion The construction of the MEL electrochemical sensor using MWCNT-modified electrodes has been demonstrated to be feasible. This MEL sensor exhibits a simple fabrication method, excellent reproducibility and stability, significant anti-interference capability, and high accuracy. Based on these advantages, its widespread application in food safety monitoring is strongly recommended.