山茱萸提取物增强皮肤对紫外线B辐射的保护作用研究

Enhanced skin protection against ultraviolet-B radiation through Cornus officinalis extract treatment

中文关键词:  山茱萸  紫外线B  基质金属蛋白酶  光损伤  抗氧化

英文关键词:Cornus officinalis  ultraviolet-B  matrix metalloproteinases  photodamage  antioxidant

中文摘要:

      目的 探讨山茱萸提取物(Cornus officinalis extract, COE)对紫外线B (ultraviolet-B, UVB)辐射引起的皮肤损伤的修复作用, 并探讨其潜在的作用机制。方法 通过检测人角化细胞(human keratinocytes cells, HaCaT)中UVB诱导的基质金属蛋白酶(matrix metalloproteinases, MMPs)及促炎细胞因子的表达, 评估COE的光保护作用, 并对其抗氧化酶活性及胶原蛋白含量进行评价。小鼠无毛皮肤在UVB辐射下暴露后, 局部应用提取物, 并测定表皮厚度、皮肤含水量及胶原mRNA的表达量。结果 在UVB照射(354.128 mJ/cm2)下, 1.00 mg/mL COE显著增强了抗氧化酶活性, 显著降低了促炎细胞因子前列腺素E2、白细胞介素1α、白细胞介素6的表达; 同时降低了MMPs的表达; 胶原蛋白含量显著增加, Ⅰ型胶原和Ⅲ型胶原蛋白的表达量分别增加13%和75%。此外, COE能够减轻UVB辐射引起的小鼠皮肤表皮增厚及胶原蛋白的丧失。同时, COE处理使丙二醛(malondialdehyde, MDA)含量降低69%, 超氧化物歧化酶(superoxide dismutase, SOD)、谷胱甘肽过氧化物酶(clutathione peroxidase, GSH-Px)活性分别升高27%和234%。对小鼠表皮mRNA表达的分析表明, 用COE处理导致Mmp1a和Mmp3 mRNA表达水平降低, Col1a1和Col3a1 mRNA水平升高。结论 COE能够有效改善UVB辐射引起的皮肤损伤, 其作用机制可能与抗氧化酶活性的增强以及促炎细胞因子和MMPs表达的抑制密切相关。本研究旨在通过小鼠和细胞模型进一步探究山茱萸对UVB诱导的皮肤损伤的光保护潜力并阐明其潜在机制, 为治疗由UVB光老化导致皮肤疾病的研究和山茱萸新药开发提供理论依据。

英文摘要:

      Objective To investigate the potential of Cornus officinalis extract (COE) to repair skin damage caused by ultraviolet-B (UVB) exposure and to elucidate the underlying mechanisms involved. Methods By detecting the expression of UVB induced matrix metalloproteinases (MMPs) and pro-inflammatory cytokines in human keratinocytes cells (HaCaT), the photoprotective effect of COE was evaluated, and its antioxidant enzyme activity and collagen content were assessed. After exposure of mouse hairless skin to UVB radiation, extracts were applied topically and epidermal thickness, skin water content and collagen mRNA expression were determined. Results Under UVB irradiation (354.128 mJ/cm2), 1 mg/mL COE significantly enhanced antioxidant enzyme activity and significantly reduced the expression of pro-inflammatory cytokines prostaglandin E2, interleukin-1 α, and interleukin-6; simultaneously reduced the expression of MMPs; the collagen content significantly increased, with the expression levels of type I collagen and type III collagen proteins increasing by 13% and 75%, respectively. In addition, COE could alleviate the thickening of mouse skin epidermis and loss of collagen caused by UVB radiation. Meanwhile, COE treatment reduced the content of malondialdehyde (MDA) by 69%, and increased the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) by 27% and 234%, respectively. Analysis of mRNA expression in mouse epidermis showed that treatment with COE resulted in a decrease in mRNA expression levels of Mmp1a and Mmp3, while mRNA levels of Col1a1 and Col3a1 increased. Conclusion COE can improve skin damage caused by UVB radiation. This improvement was associated with increased activity of antioxidant enzymes in cells, as well as reduced expression of pro-inflammatory cytokines and MMPs. The aim of this study is to further investigate the photoprotective potential of Cornus officinalis against UVB induced skin damage through mouse and cell models, and elucidate its potential mechanisms, providing a theoretical basis for the treatment of skin diseases caused by UVB photoaging and the development of new drugs from Cornus officinalis.