双乙酸钠在湿米粉中对唐菖蒲伯克霍尔德氏菌生长及产毒的影响

Effects of sodium diacetate on the growth and bongkrekic acid production of Burkholderia gladioli in wet rice noodles

中文关键词:  双乙酸钠  唐菖蒲伯克霍尔德氏菌  椰毒假单胞菌  湿米粉  米酵菌酸

英文关键词:sodium diacetate  Burkholderia gladioli  Pseudomonas cocovenenans  wet rice noodles  bongkrekic acid

中文摘要:

      目的 了解双乙酸钠对唐菖蒲伯克霍尔德氏菌在实验室培养体系及湿米粉培养体系中的生长及产毒的影响。方法 以10株自湿米粉样品及其大米原料中分离得到的和菌种保藏中心购买的唐菖蒲伯克霍尔德氏菌菌株为研究对象, 接种至添加了不同比例的双乙酸钠的培养基中, 研究其在培养基中生长及产毒情况。结果 10株唐菖蒲伯克霍尔德氏菌在BHI肉汤中生长情况一致。双乙酸钠对不同菌株抑制作用一致, 当BHI肉汤中双乙酸钠添加量>0.1%时, 10株唐菖蒲伯克霍尔德氏菌的生长均明显被抑制, 添加量达到0.2%时, 没有菌生长。在BHI半固体培养基及湿米粉培养基中, 双乙酸钠添加量超过0.1%时, 唐菖蒲伯克霍尔德氏菌的生长显著减弱甚至被完全抑制, 米酵菌酸的产量也大大减少。结论 双乙酸钠能有效抑制唐菖蒲伯克霍尔德氏菌的生长和降低米酵菌酸的产生, 有望成为安全有效且可作用于该菌的防腐剂或复配防腐剂之一, 以行业更容易接受的成本来降低唐菖蒲伯克霍尔德氏菌污染风险。

英文摘要:

      Objective To understand the effect of sodium diacetate on the growth and toxin production of Burkholderia gladioli in fresh wet rice noodles medium. Methods A total of 10 Burkholderia gladioli strains isolated from wet rice noodles samples and rice raw materials and purchased by the strain preservation center were used as research objects. The strains were inoculated into the medium supplemented with different proportions of sodium diacetate to study the growth and toxin production of the strains in the medium. Results The growth of 10 strains of Burkholderia gladioli in BHI broth was consistent. Sodium diacetate had consistent inhibitory effects on different bacterial strains. When the addition of sodium diacetate in BHI broth was more than 0.1%, the growth of 10 strains of Burkholderia gladioli was significantly inhibited, and there was no bacterial growth when the addition reached 0.2%. In BHI semi-solid medium and wet rice noodles medium, when sodium diacetate was added more than 0.1%, the growth of Burkholderia gladioli was significantly weakened or even completely inhibited, and the production of bongkrekic acid was also greatly reduced. Conclusions Sodium diacetate can effectively inhibit the growth of Burkholderia gladioli and reduce the production of bongkrekic acid. It is expected to become a safe and effective preservative or compound preservative that can act on Burkholderia gladioli, reducing the risk of contamination by Burkholderia gladioli at a more affordable cost in the industry.