一测多评法测定三大道地产区金银花中5种咖啡酰奎宁酸含量

Determination of the content of 5 kinds of caffeoylquinic acid in Lonicera japonica Thunb. from Sandao real estate area by quantitative analysis of multi-components by single marker

中文关键词:  金银花  一测多评  咖啡酰奎宁酸  质量控制

英文关键词:Lonicera japonica Thunb.  quantitative analysis of multi-components by single marker  caffeoylquinic acid  quality control

中文摘要:

      目的 建立一测多评法同时测定三大道地产区金银花药材中新绿原酸、绿原酸、异绿原酸A、异绿原酸B、异绿原酸C 5种成分的含量。方法 采用Phenomenex Synergi Hydro-RP80A (250 mm×4.6 mm, 4 μm)色谱柱, 以乙腈(A)-0.1%磷酸水溶液(B)为流动相, 检测波长327 nm, 柱温20 ℃, 流速0.7 mL/min; 进样量2 μL。结果 新绿原酸、绿原酸、异绿原酸B、异绿原酸A、异绿原酸C的线性范围分别为0.41~16.40、7.22~289.00、0.21~8.50、1.65~66.00、0.37~14.80 μg/mL (r≥0.9993), 平均回收率为95.25%~100.42%, 精密度、重复性、稳定性相对标准偏差(relative standard deviation, RSD)均小于等于2.0%; 利用外标法测25批样品中新绿原酸、绿原酸、异绿原酸A、异绿原酸B、异绿原酸C的含量, 其测定值与一测多评法计算值无显著性差异。结论 本方法同时测定金银花中5个化合物含量准确可靠且方法简便, 解决了金银花质量控制中缺乏对照品这一瓶颈问题。不同产地的金银花中咖啡酰奎宁酸类的含量差别较大。本研究为金银花的鉴别、品质评价、制定质量标准等提供了重要的依据。

英文摘要:

      Objective To establish a method to simultaneously determine the content of 5 kinds of components of Lonicera japonica Thunb. herbs from Sandao real estate area (neochlorogenic acid, chlorogenic acid, isochlorogenic acid A, isochlorogenic acid B and isochlorogenic acid C) by quantitative analysis of multi-components by single marker. Methods Phenomenex Synergi Hydro-RP80A (250 mm×4.6 mm, 4 μm) column was used with acetonitrile (A)-0.1% phosphoric acid aqueous solution (B) as mobile phase, the detection wavelength was 327 nm, the column temperature was 20 ℃, and the flow rate was 0.7 mL/min. The injection volume was 2 μL. Results The linear ranges of neochlorogenic acid, chlorogenic acid, isochlorogenic acid B, isochlorogenic acid A and isochlorogenic acid C were 0.41–16.40, 7.22–289.00, 0.21–8.50, 1.65–66.00 and 0.37–14.80 μg/mL (r≥0.9993), respectively. The average recovery rate was 95.25%–100.42%, and the relative standard deviation (RSD) of precision, repeatability and stability were less than 2.0%. The content of neochlorogenic acid, chlorogenic acid, isochlorogenic acid A, isochlorogenic acid B and isochlorogenic acid C in 25 batches of samples was determined by external standard method, and there was no significant difference between the measured values and the calculated values of quantitative analysis of multi-components by single marker method. Conclusion This method is accurate, reliable and simple for the simultaneous determination of the content of 5 kinds of compounds in Lonicera japonica Thunb., which solves the bottleneck problem of lack of reference substances in the quality control of Lonicera japonica Thunb.. The content of caffeoylquinic acid in Lonicera japonica Thunb. from different origins varies greatly, and it is necessary to further strengthen the processing of Lonicera japonica Thunb.. This study provides an important basis for the identification, quality evaluation and formulation of quality standards of Lonicera japonica Thunb..