喹啉铜在桃中的残留行为及膳食风险评估

Residue behavior and dietary risk assessment of oxine-copper in Prunus persica

中文关键词:  喹啉铜  桃  残留  膳食风险评估

英文关键词:oxine-copper  Prunus persica  residues  dietary risk assessment

中文摘要:

      目的 研究喹啉铜在桃中的残留及其膳食风险评估。方法 样品经10%冰乙酸-乙腈匀浆提取, 盐析离心, 采用超高效液相色谱-串联质谱法(ultra performance liquid chromatography-tandem mass spectrometry, UPLC-MS/MS)分析检测, 外标法定量。结果 在0.0025~0.2500 mg/L范围内呈良好线性关系, 相关系数为0.999; 添加浓度为0.01~1.00 mg/kg时, 喹啉铜的平均回收率范围为86%~102%, 相对标准偏差为1.4%~6.0%, 定量限为0.01 mg/kg。喹啉铜在桃中的残留消解动态规律符合一级动力学反应模型, 宁夏、北京市、山东省、四川省三地喹啉铜在桃中的消解半衰期分别为13.9、9.9、7.7、23.1 d, 决定系数为0.635~0.948, 施药14 d后, 喹啉铜在桃样品中最大残留量为0.920 mg/kg。膳食风险评估表明, 施药14 d后, 一般人群膳食风险概率均远小100%。结论 该方法简便、准确、灵敏度高, 可用于桃中喹啉铜的残留检测。最终残留量试验显示, 喹啉铜残留量未超过已制定的最大残留限量, 对一般人群健康不会产生不可接受的风险。

英文摘要:

      Objective To study the residual behavior and dietary risk assessment of oxine-copper in Prunus persica. Methods The samples were extracted by homogenate with acetonitrile-10% acetic solution, centrifuged after salting out, detected by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), and quantified by external standard method. Results There was a good linear relationship in the range of 0.0025 to 0.2500 mg/L, and the correlation coefficients was 0.999; in the range of 0.01 to 1.00 mg/kg; the average recoveries of oxine-copper in Prunus persica ranged from 86% to 102%, with the relative standard deviation was 1.4% to 6.0%, and the limit of quantification was 0.01 mg/kg. The dissipation process of oxine-copper in Prunus persica followed a first-order kinetic model. The dissipation half-lives of oxine-copper in Prunus persica from Ningxia, Beijing, Shandong and Sichuan were 13.9, 9.9, 7.7 and 23.1 days, respectively, and the correlation coefficients ranged from 0.635 to 0.948, after 14 days of application, the maximum residual amount of oxine-copper in Prunus persica samples was 0.920 mg/kg. The dietary risk assessment showed that after 14 d of application, the probability of dietary intake risk in the general population was far less than 100%. Conclusion This method is simple, accurate, and highly sensitive, and is suitable for the residual detection oxine-copper in Prunus persica. The final residue tests show that the amount of copper quinoline residue does not exceed the established maximum residue limits, and does not pose an unacceptable risk to the health of the general population.