干制水果中金黄色葡萄球菌多种鉴定方法的比较

Comparison of Multiple Identification Methods for Staphylococcus aureus in Dried Fruits

中文关键词:  特色干制水果  金黄色葡萄球菌  鉴定

英文关键词:Characteristic dried fruit  Staphylococcus aureus  identi?cation

中文摘要:

      目的 比较生化鉴定、质谱仪鉴定、分子生物学鉴定3类实验方法鉴定金黄色葡萄球菌结果的异同。方法 对干制水果中的金黄色葡萄球菌进行分离，分离的典型菌株通过生物化学（VITEK 2-compact）、质谱（VITEK MS）及分子生物学（实时荧光定量PCR Real-time Fluorescent Quantitative PCR） 3类方法进行鉴定，并比较3类方法实验的鉴定结果。结果 三类鉴定方法对金黄色葡萄球菌均鉴定到种水平，但在鉴定效率和时限有一定差异，质谱鉴定的结果更准确，且时效性更高。结论 本研究分析不同鉴定方法优缺点，将传统微生物检测方法与现代技术联用，在传统检测方式的增菌、分离为后续鉴定提供基础，在此之上结合不同原理的先进鉴定技术，形成一套完整的检测流程。这种联用模式可相互验证结果，提高检测准确性，为建立更可靠的特色干制水果微生物检测体系提供新思路。

英文摘要:

      Objective To compare the similarities and differences in the identification results of Staphylococcus aureus analyzed by three experimental methods: biochemical identification, mass spectrometer identification, and molecular biological identification. Methods Staphylococcus aureus in dried fruits was isolated. The isolated typical strains were identified and compared through three approaches: biochemistry (VITEK 2-compact), mass spectrometer (VITEK MS), and molecular biology (Real-time Fluorescent Quantitative PCR), and the identification results of the three types of experiments were compared. Results All three identification methods can identify Staphylococcus aureus to the species level, but there are certain differences in efficiency and time required. Mass spectrometry yields more accurate results with higher timeliness.. Conclusion This paper analyzes the advantages and disadvantages of different identification methods, combines traditional methods with modern technologies. The enrichment and isolation in traditional detection methods provide a basis for subsequent identification. On this basis, advanced identification technologies with different principles are integrated to form a complete detection process. This combined mode can mutually verify the results, improve the detection accuracy, and provide new ideas for establishing a more reliable microbiological detection system for characteristic dried fruits.