负载植物乳植杆菌CXG9的可食用复合薄膜的制备表征及抗菌特性研究

Characterization and antibacterial activity of edible composite films embedded with Lactiplantibacillus plantarum CXG9

中文关键词:  明胶  玉米淀粉  植物乳植杆菌CXG9  可食用薄膜  抑菌活性

英文关键词:gelatin  corn starch  Lactiplantibacillus plantarum CXG9  edible film  antibacterial activity

中文摘要:

      目的 制备负载活性植物乳植杆菌CXG9的可食用明胶-玉米淀粉生物基复合薄膜, 赋予薄膜抗菌活性和优良性能。方法 以明胶和玉米淀粉混合薄膜为基材, 将植物乳植杆菌CXG9离心所得菌泥混入明胶-玉米淀粉混合液中, 通过溶液浇铸法制备活菌可食用复合薄膜。通过对薄膜性能的表征和体外抗菌实验, 研究植物乳植杆菌CXG9的加入对明胶-玉米淀粉薄膜性能的影响。结果 负载植物乳植杆菌CXG9的明胶-玉米淀粉复合薄膜断裂伸长率提高35.51%, 水分含量下降2.55%, 水蒸气透过率下降11.2%, 说明负载植物乳植杆菌CXG9薄膜的紫外线阻隔能力、水蒸气透过率、断裂伸长率和热稳定性都有所改善。活菌的加入赋予薄膜抗菌性能, 对单增李斯特菌、志贺氏菌、大肠杆菌、金黄色葡萄球菌、鼠伤寒沙门氏菌和铜绿假单胞菌都有不同的抑制程度。结论 本研究选用明胶-玉米淀粉为基质, 成功制备出负载了植物乳植杆菌CXG9的明胶-CXG9-玉米淀粉抗菌薄膜, 降低了传统包装材料对环境的负担, 为食品包装材料的开发开辟了新途径。

英文摘要:

      Objective To prepare edible gelatin-corn starch bio-based composite film loaded with lactive Lactiplantibacillus plantarum CXG9, and endow the film with antibacterial activity and excellent film properties. Methods Using gelatin and corn starch mixed film as substrate, the bacterial mud obtained by Lactiplantibacillus plantarum CXG9 centrifugation was mixed into gelatin-corn starch mixture, and the living bacteria edible composite film was prepared by solution casting method. By measuring the characterization of the film and its antibacterial activity in vitro, the effects of Lactiplantibacillus plantarum CXG9 on the properties of gelatin-corn starch film were studied. Results The elongation at break of the gelatin-corn starch composite film loaded with Lactiplantibacillus plantarum CXG9 increased by 35.51%, the moisture content decreased by 2.55%, and the water vapor permeability also decreased by 11.2%. This indicated that the ultraviolet barrier ability, water vapor permeability, elongation at break, and thermal stability of the film loaded with Lactiplantibacillus plantarum CXG9 had all been improved. On the other hand, the addition of live bacteria gave the film antibacterial properties, and it had different degrees of inhibition on Listeria monocytogenes, Shigella, Escherichia coli, Staphylococcus aureus, Salmonella typhimurium and Pseudomonas aeruginosa. Conclusion In this study, gelatin-CXG9-corn starch antibacterial film loaded with Lactiplantibacillus plantarum CXG9 is successfully prepared by using gelatin-corn starch as substrate, which reduces the burden of traditional packaging materials on the environment and opens up a new way for the development of food packaging field.