glnA基因敲除对土壤杆菌产可得然胶的影响

Effects of glnA gene knockout on the production of available curdlan by Agrobacterium

中文关键词:  可得然胶  土壤杆菌  基因敲除

英文关键词:curdlan  Agrobacterium  gene knockout  glnA gene

中文摘要:

      目的 探究glnA基因对土壤杆菌产可得然胶的影响。方法 构建glnA基因敲除株ΔglnA菌株, 分析ΔglnA菌株生长代谢情况和产生的可得然胶在产量、凝胶性质和红外结构的变化, 并通过实时荧光定量聚合酶链式反应的方法探究可得然胶合成相关基因的表达量。结果 ΔglnA菌株在发酵过程中氨基氮消耗情况与CGMCC11546一致, 在蔗糖消耗方面, ΔglnA菌株与CGMCC11546在12 h之前无明显差别, 但在15 h之后出现明显差异, 蔗糖消耗比CGMCC11546明显降低。ΔglnA菌株可得然胶产量约4 g/L, 相对于CGMCC11546降低了60%。结论 glnA基因不影响菌体生长和可得然胶结构, 但可以使可得然胶的合成减少。本研究通过对glnA基因进行敲除, 研究其对可得然胶合成的影响, 为后续提高可得然胶产量提供一些分子生物学基础。

英文摘要:

      Objective To explore the effects of glnA gene on the production of curdlan by Agrobacterium. Methods A glnA gene knockout strain ΔglnA was constructed in this paper. The growth and metabolism of ΔglnA strain and the changes in yield, gel properties and infrared structure of the obtained gum were analyzed, and the expression of genes related to the synthesis of curdlan was explored by quantitative real-time polymerase chain reaction. Results The amino nitrogen consumption of the ΔglnA strain during fermentation was consistent with that of CGMCC11546. In terms of sucrose consumption, there was no significant difference before 12 h, but there was a significant difference between the ΔglnA strain and CGMCC11546 after 15 h, and sucrose consumption was significantly reduced compared to CGMCC11546. The ΔglnA strain could produce approximately 4 g/L of natural gum, which was 60% lower than CGMCC11546. Conclusion The glnA gene does not affect bacterial growth or the structure of collagen, but it can reduce the synthesis of collagen. This study provides insights into the role of glnA in curdlan production and lays a molecular biological foundation for further efforts to enhance curdlan yield.