高效液相色谱法测定食品接触材料中14种紫外线吸收剂迁移量

Determination of the migration of 14 kinds of ultraviolet absorbers in food contact materials by high performance liquid chromatography

中文关键词:  食品接触材料  高效液相色谱  迁移量  紫外线吸收剂

英文关键词:food contact material  high performance liquid chromatography  migration  ultraviolet absorber

中文摘要:

      目的 建立高效液相色谱法检测食品接触材料及制品中14种紫外线吸收剂在水性食品模拟物和化学替代溶剂(95%乙醇水溶液和异辛烷)中迁移量的方法。方法 迁移到水性食品模拟物中的紫外线吸收剂用正己烷涡旋振荡提取两次, 合并的提取液减压旋转蒸发至干, 用四氢呋喃复溶; 迁移至化学替代溶剂中的紫外线吸收剂, 旋转蒸发至干, 用四氢呋喃复溶。选用C8色谱柱分离, 乙腈和水为流动相, 梯度洗脱。紫外检测器在300 nm波长下检测。结果 14种紫外线吸收剂方法的检出限可以达到0.008 mg/L, 定量限可达到0.020 mg/L。14种目标物在0.2~5.0 mg/L线性范围内相关系数达到0.999以上。在食品模拟物或化学替代溶剂中加添加3个水平紫外线吸收剂(0.02、0.05和0.50 mg/L), 6次重复实验, 结果显示各个化合物在各水平下的回收率在82.4%~108.0%范围内, 相对标准偏差小于10%。结论 本方法准确、高效、简便, 可用于同步检测水性模拟物与化学替代溶剂中14种紫外线吸收剂的迁移量。

英文摘要:

      Objective To establish a method for the determination of the migration of 14 kinds of ultraviolet absorbers in aqueous food simulants and chemical alternative solvents (95% ethanol aqueous solution and isooctane) by high performance liquid chromatography. Methods The ultraviolet absorbers migrated into the aqueous food simulants were extracted twice with n-hexane by vortexing and shaking, and the combined extract was evaporated to dryness under reduced pressure and re-dissolved in tetrahydrofuran. The ultraviolet absorbers migrated into the chemical alternative solvents were evaporated to dryness and re-dissolved in tetrahydrofuran. A C8 column was used for separation, with acetonitrile and water as the mobile phase and gradient elution. Detection was performed using a ultraviolet detector at a wavelength of 300 nm. Results The limits of detection of 14 kinds of ultraviolet absorbers were as low as 0.008 mg/L, and the limits of quantitation were 0.020 mg/L. The correlation coefficients of the 14 kinds of target compounds were above 0.999 within the linear range of 0.20–5.0 mg/L. The 3 levels of ultraviolet absorbers (0.02, 0.05 and 0.50 mg/L) were added to the food simulants or chemical alternative solvents, and 6 replicate experiments were conducted. The results showed that the recovery rates of each compound at each level were within the range of 82.4%–108.0%, and the relative standard deviations were less than 10%. Conclusion This method is accurate, efficient and simple, and can be used to simultaneously determine the migration amounts of 14 kinds of ultraviolet absorbers in aqueous simulants and chemical alternative solvents.