基于新方法检测天然鱼肝油中维生素A和不饱和脂肪酸

Identification of vitamin A and unsaturated fatty acid in natural cod liver oil based on a new method

中文关键词:  鱼肝油  维生素A  不饱和脂肪酸  定量检测

英文关键词:cod liver oil  vitamin A  unsaturated fatty acids  quantitative determination

中文摘要:

      目的 建立新方法简便鉴别天然鱼肝油中维生素A (vitamin A, VA)和不饱和脂肪酸的含量。方法 将鱼肝油样品经皂化后注入高效液相色谱系统, 检测VA的含量。将鱼肝油及其辅料皂化后注入气相色谱系统, 分析其中亚油酸、二十碳五烯酸(eicosapentaenoic acid, EPA)和二十二碳六烯酸(docosahexaenoic acid, DHA)定位以及所占百分比, 将鱼油与植物油进行鉴别与区分。结果 在新方法下, VA浓度在30.00~1500.00 IU/mL范围内线性关系良好(r2=0.9999); 平均回收率为96.1%; 仪器精密度的相对标准偏差为0.18%, 方法精密度的相对标准偏差为1.13%, 中间精密度的相对标准偏差为0.98%。各类植物油与鱼油及鱼肝油中脂肪酸含量差异显著, 鱼肝油、鱼油中EPA、DHA含量丰富, 亚油酸含量少; 玉米油、菜籽油、橄榄油中均不含EPA和DHA, 但亚油酸含量丰富。结论 本研究建立的检测方法简便准确, 可用于鱼肝油中VA和不饱和脂肪酸含量测定, 为制定理想的质量标准奠定基础。

英文摘要:

      Objective To establish a new method for simple identification of vitamin A (VA) and unsaturated fatty acids in natural cod liver oil. Methods The cod liver oil sample was saponified and injected into a high-performance liquid chromatography system to detect the content of VA. After saponification of cod liver oil and its excipients, injected them into a gas chromatography system to analyze the localization and percentage of linoleic acid, eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA), and distinguish fish oil from vegetable oil. Results Under the new method, the linear relationship of VA concentration was good within the range of 30.00–1500.00 IU/mL (r2=0.9999); the average recovery rate was 96.1%. The relative standard deviation of instrument precision was 0.18%, the relative standard deviation of method precision was 1.13%, and the relative standard deviation of intermediate precision was 0.98%. There was a significant difference in the content of fatty acids between vegetable oils, fish oil and cod liver oil. Cod liver oil and fish oil were rich in EPA and DHA, while linoleic acid was low. Corn oil, rapeseed oil and olive oil did not contain EPA and DHA, but they were rich in linoleic acid. Conclusion The detection method establish in this article is simple and accurate, and can be used for the determination of VA and unsaturated fatty acid content in cod liver oil, laying the foundation for the development of ideal quality standards.