白芍煎煮浓缩后苯甲酸的含量变化研究

Study on the change of benzoic acid content in water extract of Paeoniae Radix Alba after decocting and concentrating

中文关键词:  白芍  苯甲酸  高效液相色谱法

英文关键词:Paeoniae Radix Alba  benzoic acid  high performance liquid chromatography, decocting and concentrating

中文摘要:

      目的 研究煎煮后白芍水浓缩液中苯甲酸含量的变化, 以及不同煎煮时间对白芍中苯甲酸含量的影响。方法 对原料进行单独和组合煎煮, 并浓缩至密度为1.15~1.20之间, 用高效液相色谱法检测煎煮浓缩后白芍水浓缩液中的苯甲酸含量。结果 未检出苯甲酸(定量限为0.01 g/kg)的白芍饮片经过单次煎煮浓缩后, 其苯甲酸含量增加至2.91~3.77 g/kg, 经过两阶段煎煮浓缩后的白芍浓缩液, 其苯甲酸含量范围为3.26~4.02 g/kg, 含有白芍的混合浓缩液中苯甲酸含量为0.542 g/kg。其余浓缩液中苯甲酸均未检出。结论 白芍经过高温煎煮后水煮浓缩液中苯甲酸含量增加, 且与煎煮时长成正比关系, 因此在制备以白芍为主要原料的食品或保健品时应注意控制煎煮温度和煎煮时长, 增加其出厂检测, 以防苯甲酸含量过高造成危害。

英文摘要:

      Objective To study the change of benzoic acid content in water extract of Paeoniae Radix Alba after decocting, and the influence of different decocting time on the content of benzoic acid. Methods The raw materials were decocted separately or in combination, and concentrate to a density of 1.15–1.20, the content of benzoic acid in the water extract after decocting and concentrating was determined by high performance liquid chromatography. Results Benzoic acid content was not detected (limit of quantification was 0.01 g/kg) in Paeoniae Radix Alba, the benzoic acid content in concentrated liquid of paeony increased to 2.91–3.77 g/kg after single decocting, and the benzoic acid content after two-stage decocting ranged from 3.26 g/kg to 4.02 g/kg. The content of benzoic acid in the mixed concentrate containing Paeoniae Radix Alba was 0.542 g/kg. Benzoic acid was not detected in others. Conclusion The content of benzoic acid in water extract of Paeoniae Radix Alba increased after high temperature decocting, and it’s proportional to time. Therefore, when preparing food or health products with Paeoniae Radix Alba as the main raw material, attention should be paid to the control of decocting temperature and time, and the factory inspection of this item should be increased to prevent the harm caused by high content of benzoic acid.