食叶草降糖大米的研制及其对α-葡萄糖苷酶的抑制活性

Development of artificial hypoglycemic rice from edible leaf grass and its inhibitory activity on α-glucosidase

中文关键词:  食叶草  降糖大米  糖尿病  α-葡萄糖苷酶抑制能力

英文关键词:edible leaf grass  artificial hypoglycemic rice  diabetes  α-glucosidase

中文摘要:

      目的 开发一种以食叶草为营养补充剂的人造降糖大米, 优化其配方并分析其对α-葡萄糖苷酶的抑制活性及感官品质。方法 采用正交实验设计优化人造降糖大米的配方参数, 以玉米直链淀粉和大米淀粉为主要原料, 食叶草为功能性添加剂。通过酶标仪检测α-葡萄糖苷酶抑制活性, 评估样品的降糖效果, 并依据感官评价方法对其进行食品感官分析。结果 食叶草水提物对α-葡萄糖苷酶的抑制率较高, 半数抑制浓度(inhibitory concentration, IC50)值为1.189 mg/mL, 接近阳性对照阿卡波糖的抑制效果。优化配方生产的食叶草人造降糖大米在感官评价中表现出色, 具有良好的风味、口感及外观。结论 食叶草具有优异的营养价值和潜在的降糖活性。基于其开发的人造降糖大米既满足糖尿病患者的特殊需求, 也为健康食品市场提供了新选择, 具有广阔的应用前景。

英文摘要:

      Objective To develop artificial hypoglycemic rice with leafy grass as a nutritional supplement, optimize its formula, and analyze its inhibitory activity on α-glucosidase and sensory quality. Methods The orthogonal experimental design was used to optimize the formula parameters of artificial rice, with corn amylose and rice starch as the main raw materials and edible leaf grass powder as the functional additive. The inhibitory activity of α-glucosidase was detected by enzyme labeling instrument to evaluate the hypoglycemic effect the samples, and the food sensory analysis was carried out according to the sensory evaluation method. Results The water extract of edible leaf grass showed a high inhibitory rate on α-glucosidase with an inhibitory concentration (IC50) value 1.189 mg/mL, which was close to the inhibitory effect of the positive control acarbose. The optimized formula for producing artificial rice from edible leaf grass performed excellently in sensory evaluation, with good flavor, texture and appearance. Conclusion Edible leaf grass has excellent nutritional value and potential hypoglycemic activity. The artificial hypoglycemic rice develops based on it not only meets the special needs of diabetes patients, but also provides a new choice for the health food market, and has broad application prospects.