微波消解-氢化物原子荧光法测定牛肉及内脏中硒含量

Determination of selenium content in beef and offal by microwave digestion-hydride atomic fluorescence spectrometry

中文关键词:  微波消解  原子荧光光谱  富硒牛肉  

英文关键词:microwave digestion  atomic fluorescence spectrometry  selenium  beef  offal

中文摘要:

      目的 建立微波消解-氢化物原子荧光光谱法(microwave digestion-hydride atomic fluorescence spectrometry, HG-AFS)测定牛肉及内脏(牛心、牛肝、牛肺)中硒含量的分析方法。方法 根据牛的饲喂量和饲喂时间两个因素, 选择体重在500 kg左右(±10 kg)西门塔尔公牛80头, 随机分成A、B、C、D 4组, A、B、C 3组作为实验组, D组为对照组, 每组20头, 采用硝酸-过氧化氢(HNO3-H2O2)微波消解牛肉及内脏样本中的硒, 硼氢化钾为还原剂, 10%盐酸为载流, 190 ℃消解。结果 硒在0~20 ng/mL质量浓度范围内呈现良好的线性关系, 相关系数(r)>0.999, A、B、C、D 4组样本的加标回收率(n=3)平均值分别为86.9%、82.4%、87.9%、83.8%。结论 该研究为富硒牛产品中硒含量的测定以及富硒功能农业的发展提供了数据支撑和参考依据。

英文摘要:

      Objective To establish an analytical method for the determination of selenium content in beef and offal (beef heart, beef liver, beef lung) using microwave digestion-hydride atomic fluorescence spectrometry (HG-AFS). Methods Based on 2 factors, namely the feeding amount and feeding time of the cattle, 80 simmental bulls weighing around 500 kg (±10 kg) were selected and randomly divided into 4 groups: A, B, C and D. Groups A, B and C served as experimental groups, while group D was the control group, with 20 cattle in each group. Selenium in beef and offal samples was digested using a nitric acid-hydrogen peroxide (HNO3-H2O2) microwave digestion method, with potassium borohydride as the reducing agent and 10% hydrochloric acid as the carrier stream, at a digestion temperature of 190 ℃. Results Selenium exhibited a good linear relationship within the mass concentration range of 0–20 ng/mL, with a correlation coefficient (r)>0.999. The average spike recovery rates (n=3) for samples A, B, C and D were 86.9%, 82.4%, 87.9% and 83.8%, respectively. Conclusion This study provides data support and a reference basis for the determination of selenium content in selenium-enriched cattle products and the development of selenium-enriched functional agriculture.