一株多重耐药性单增李斯特菌全基因组分析

Genomic characteristics analysis of a multidrug-resistant foodborne Listeria monocytogenes strain

中文关键词:  单增李斯特菌  毒力基因  耐药表型  耐药基因

英文关键词:Listeria monocytogenes  virulence genes  resistance phenotype  resistance genes

中文摘要:

      目的 从甘肃省市售冷冻鸡肉中分离到一株单增李斯特菌, 分析其血清型、耐药表型及其基因组特征。方法 玻片凝集法进行血清学鉴定, 微量肉汤法分析菌株对8种抗生素敏感性, 测序分析其基因组特征。结果 该菌株经过16s rDNA种属鉴定为单增李斯特菌, 为1/2a血清型, 系统发育谱系Ⅱ、亚系SL 155、克隆群CC 155、MLST 705、cgMLST 13240。对氨苄西林、青霉素、万古霉素、美罗培南4种抗生素敏感, 对红霉素、环丙沙星、四环素和复方新诺明4种抗生素多重耐药。同时携带aph(3')-III、ant(6)-Ia、dfrG、fosX、lnu(B)、lsa(E)、erm(B)、tet(S)和tet(M) 9种耐药基因, 携带LIPI-1的5个毒力基因, prfA基因缺失, 携带12种内化素基因, 未检测到inlA基因携带提前终止密码子, 不携带LIPI-3和LIPI-4。结论 该菌携带丰富的毒力基因和耐药基因, 其耐药表型与耐药基因基本一致, 表现出多重耐药, 为李斯特菌病的防治提供参考依据。

英文摘要:

      Objective To isolate a strain of Listeria monocytogenes from frozen chicken meat sold in Gansu Province and analyze its serotype, antibiotic resistance phenotype, and genomic characteristics. Methods The slide agglutination method was used for serological identification, the broth microdilution method was used to analyze the sensitivity of the strain to 8 kinds of antibiotics, and the genomic characteristics were analyzed by sequencing. Results The strain was identified as Listeria monocytogenes by 16S rDNA species identification, with a 1/2a serotype, phylogenetic lineage II, subline SL 155, and clone CC 155 MLST 705, cgMLST 13240. Sensitive to 4 kinds of antibiotics including ampicillin, penicillin, vancomycin, and meropenem, and multidrug-resistant to 4 kinds of antibiotics including erythromycin, ciprofloxacin, tetracycline, and compound sulfamethoxazole. Simultaneously carrying aph(3')-III, ant(6)-Ia, dfrG, fosX, lnu(B), lsa(E), erm(B), tet(S) and tet(M) 9 kinds of resistance genes, carrying 5 virulence genes of LIPI-1, prfA gene deletion, carrying 12 kinds of internalization genes, no premature stop codon of inlA gene carrying, no LIPI-3, and LIPI-4. Conclusions The Listeria monocytogenes carries abundant virulence genes and resistance genes, and its resistance phenotype is basically consistent with the resistance genes, showing multiple drug resistance, providing a reference for the prevention and treatment of listeriosis.