基于竞争抑制免疫层析技术检测香蕉中新烟碱类农药的研究

Research on the detection of neonicotinoid pesticides in Musa nana Lour. based on competitive inhibition immunochromatographic technology

中文关键词:  免疫层析技术  香蕉  新烟碱农药  快速检测

英文关键词:immunochromatography technology  Musa nana Lour.  neonicotinoid pesticide  rapid detection

中文摘要:

      目的 建立一种同时检测香蕉中3种高不合格率新烟碱类农药的竞争抑制免疫层析快速检测方法。方法 样品通过20%甲醇-0.05%吐温-0.02 mol/L 磷酸盐缓冲(phosphate-buffered saline, PBS)溶液提取, 8000 r/min离心5 min, 分别取100 μL上清液按照稀释倍数稀释, 再取稀释后的待测液100 μL于样品孔(S)中, 等待反应时间8 min后判读结果。结果 该方法吡虫啉、噻虫胺、噻虫嗪检出限分别为0.050、0.020、0.002 mg/kg, 样品稀释倍数分别为2倍、不稀释、5倍。经评价, 相对准确度分别为96.7%、98.3%、100.0%, 与液相色谱-质谱法检测香蕉中吡虫啉、噻虫胺、噻虫嗪结果一致。结论 此快速检测方法具有操作简单、检测快速、特异性强、仪器便携、结果可视化等特点, 适合于香蕉中农药残留的现场快速检测。

英文摘要:

      Objective To establish a rapid competitive inhibition immunochromatographic detection method for the simultaneous determination of 3 kinds of neonicotinoid pesticides with a high non-compliance rate in Musa nana Lour.. Methods The samples were extracted using a solution composed of 20% methanol, 0.05% Tween, and 0.02 mol/L phosphate-buffered saline (PBS). After centrifugation at 8000 r/min for 5 min, 100 μL of the supernatant was taken and diluted according to specific dilution ratios. Subsequently, 100 μL of the diluted sample solution was added to the sample well (S). The results were read after 8 min reaction period. Results The limits of detection for imidacloprid, clothianidin, and thiamethoxam were 0.050, 0.020 and 0.002 mg/kg, respectively, with corresponding sample dilution factors of 2-fold, no dilution, and 5-fold. Upon evaluation, the relative accuracies were determined to be 96.7%, 98.3% and 100.0%, respectively, which were in agreement with the results obtained by the liquid chromatography-mass spectrometry method for the detection of these pesticides in Musa nana Lour.. Conclusion This rapid detection method exhibits characteristics such as simplicity in operation, rapidity in detection, and portability of the instrument, rendering it suitable for on-site and rapid screening of pesticide residues in Musa nana Lour..