2023年扬州市市场和餐饮中副溶血弧菌的病原特征分析

Analysis of pathogenic characteristics of Vibrio parahaemolyticus isolated in the markets and catering of Yangzhou in 2023

中文关键词:  副溶血弧菌  分离鉴定  运动性  溶血活性  耐药表型

英文关键词:Vibrio parahaemolyticus  isolation and identification  motility  hemolytic activity  drug resistance phenotype

中文摘要:

      目的 了解扬州市淡水产品、海产品及熟食餐饮中副溶血弧菌的流行情况、毒力表型和耐药表型。方法 在扬州市场9个采样点采集淡水产品、海产品和熟食样品, 进行副溶血弧菌分离鉴定, 并测定分离株的运动性、溶血活性和耐药表型。结果 2023年在扬州市共采集289份样品, 分离到63株副溶血弧菌, 总分离率为21.80%, 其中, 淡水产品分离率为45.35% (39/86), 海产品分离率为28.75% (23/80), 熟食样品分离率为0.81% (1/123), 表明扬州地区淡水产品分离率高于海水市场。淡水产品分离株中具有较强运动能力的菌株比例为30.77%, 高于海产品的17.39%; 而具有较强溶血活性的菌株比例在淡水产品为53.85%, 略低于海产品的60.87%。耐药表型的结果显示, 副溶血弧菌分离株对氨苄西林和头孢唑林的耐药率为100.00%, 对头孢他啶、四环素、环丙沙星、氯霉素、阿米卡星、美罗培南、庆大霉素7种抗生素均不耐药。结论 扬州市场副溶血弧菌检出率较高, 揭示了不同来源分离株在毒力和耐药性方面的差异, 为相关部门的监管和防治提供科学依据。

英文摘要:

      Objective To investigate the prevalence, virulence phenotypes, and drug resistance phenotype of Vibrio parahaemolyticus in freshwater food, seafood, and ready-to-eat food in Yangzhou. Methods Freshwater food, seafood and ready-to-eat food samples were collected from 9 sampling points in Yangzhou markets for isolation and identification of Vibrio parahaemolyticus. The motility, hemolytic activity, and drug resistance phenotype of the isolates were analyzed. Results In 2023, a total of 289 samples were collected in the Yangzhou market, from which 63 strains of Vibrio parahaemolyticus were isolated, with an isolation rate of 21.80%. Among them, the isolation rate of freshwater food was 45.35% (39/86), seafood 28.75% (23/80), and ready-to-eat samples 0.81% (1/123), indicating a higher isolation rate in freshwater food compared to seafood in the markets of Yangzhou. The proportion of highly motility strains in freshwater food isolates was 30.77%, higher than that of the seafood isolates (17.39%). However, the proportion of strains with high hemolytic activity was 53.85% in freshwater food isolates, slightly lower than the 60.87% in seafood isolates. Antibiotic resistance profiling showed that all Vibrio parahaemolyticus isolates were resistant to ampicillin and cefazolin, while they were resistant to ceftazidime, tetracycline, ciprofloxacin, chloramphenicol, amikacin, meropenem and gentamicin. Conclusion This study highlights a high detection rate of Vibrio parahaemolyticus in Yangzhou markets and reveals differences in virulence and antibiotic resistance among isolates from different sources, offering valuable insights for monitoring and controlling the pathogen.