2023—2024年徐州市空肠弯曲菌耐药及毒力基因分析

Analysis of drug resistance and virulence genes of Campylobacter jejuni in Xuzhou City from 2023 to 2024

中文关键词:  空肠弯曲菌  全基因组测序  血清型  多重耐药

英文关键词:Campylobacter jejuni  whole genome sequencing  serotype  multi-drug resistance

中文摘要:

      目的 探究2023—2024年徐州市空肠弯曲菌的耐药表型、耐药基因及毒力基因携带情况、血清型、序列类型(sequence type, ST)分型。方法 对2023—2024年徐州市分离的空肠弯曲菌进行全基因组测序分析了解毒力基因、耐药基因、多位点序列分型等分子特征, 通过实时荧光定量聚合酶链式反应(polymerase chain reaction, PCR)法了解格林-巴利综合征相关的血清型, 通过琼脂稀释法了解其耐药表型。结果 48株空肠弯曲菌共有24个ST型, ST137最多, 占比18.75% (9/48), 有15株菌检出格林-巴利综合征相关的血清型, 共检出24种耐药基因和121种毒力基因。耐药率排前3位的抗生素分别是萘啶酸(100.00%)、环丙沙星(100.00%)、四环素(97.92%), 对红霉素100.0%敏感, 多重耐药率达到62.50% (30/48)。结论 徐州地区空肠弯曲菌具有多重耐药率高、部分耐药基因携带率高等特征, 并且毒力基因携带率高, ST型较多, 表现出较高的遗传多样性。

英文摘要:

      Objective To understand the drug resistance phenotypes, , carriage of drug resistance genes and virulence genes, serotypes and sequence type (ST) classification of Campylobacter jejuni in Xuzhou from 2023 to 2024. Methods Whole genome sequencing was conducted on Campylobacter jejuni isolated from Xuzhou City in 2023—2024 to understand the molecular characteristics such as virulence genes, drug resistance genes, and multilocus sequence typing. The serotypes related to Guillain-Barré syndrome were identified by real-time fluorescent quantitative polymerase chain reaction (PCR), and the drug resistance phenotypes were determined by agar dilution method. Results Forty-eight strains of Campylobacter jejuni were classified into 24 ST types. ST137 was the most numerous, accounted for 18.75% (9/48). Fifteen strains were found to have serotypes related to Guillain-Barré syndrome. A total of 24 drug resistance genes and 121 virulence genes were detected. The top 3 antibiotics with the highest resistance rates were nalidixic acid (100.00%), ciprofloxacin (100.00%), and tetracycline (97.92%). They were 100.0% sensitive to erythromycin, and the multi-drug resistance rate reached 62.50% (30/48). Conclusion Campylobacter jejuni in Xuzhou area is characterized by a high rate of multi-drug resistance, a relatively high carrying rate of some drug resistance genes, a high carrying rate of virulence genes, a large number of sequence types (ST types), and shows relatively high genetic diversity.