高效液相色谱-紫外检测器法测定食品中低聚木糖含量

Determination of xylo-oligosaccharides content in food by high performance liquid chromatography-ultraviolet detector

中文关键词:  高效液相色谱  紫外检测器  低聚木糖  前处理方法  色谱条件

英文关键词:high performance liquid chromatography-ultraviolet detector  xylo-oligosaccharide  pretreatment method  chromatographic condition

中文摘要:

      目的 建立高效液相色谱-紫外检测器法测定食品中低聚木糖含量的方法。方法 前处理方法中考察称样量, 水解时间, 衍生时间, 净化次数; 色谱条件对梯度洗脱和等度洗脱进行了比较, 同时考察了柱温和检测波长。结果 最佳的称样量为2.0 g, 最佳的水解时间为100 min, 最佳的衍生时间为100 min, 最佳净化次数为3次, 流动相采取梯度洗脱方法最佳, 最佳的柱温为30 ℃, 最佳的检测波长为250 nm。结论 该方法测定食品中低聚木糖具有良好的准确性和灵敏度, 本研究为食品中开发低聚木糖含量测定方法提供数据支持。

英文摘要:

      Objective To establish a high performance liquid chromatography-ultraviolet detector method for the determination of xylo-oligosaccharide content in food. Methods The sample size, hydrolysis time, derivatization time and purification times in the pre-treatment method were investigated. Gradient elution was compared with isocratic elution to fix the chromatographic conditions. Moreover, the detection wavelength and column temperature were obtained at the same time. Results The optimized condition was as follows: Weighing sample 2.0 g; hydrolysis time 100 min; derivation time 100 min; purification for 3 times; mobile gradient elution was employed, column temperature 30 ℃; detection wavelength 250 nm. Conclusion This method has good accuracy and sensitivity for the determination of xylo-oligosaccharides in food. This study provides data support for the development of a method for the determination of xylo-oligosaccharides in food.