基于超高效液相色谱-串联四极杆/轨道阱高分辨质谱法分析桔梗茎叶代谢物

Metabolite composition analysis of leaves and stems of Platycodon grandiflorus based on ultra performance liquid chromatography-Quadrupole/Orbitrap high-resolution mass spectrometry

中文关键词:  紫花桔梗  茎叶  化学成分  高分辨质谱法

英文关键词:purple flowered Platycodon grandiflorum  leaves and stems  chemical composition  ultra performance liquid chromatography-Quadrupole/Orbitrap high-resolution mass spectrometry

中文摘要:

      目的 基于超高效液相色谱-串联四极杆/轨道阱高分辨质谱法(ultra performance liquid chromatography-Quadrupole/Orbitrap high-resolution mass spectrometry, UPLC-Q/Orbitrap HRMS)分析桔梗茎叶中代谢物的种类和含量。方法 本研究以来源不同的两年生紫花桔梗茎叶为实验材料, 利用UPLC-Q/Orbitrap HRMS, 在负离子模式下, 采用轨道阱一级全扫描和数据依赖型离子阱二级扫描对桔梗茎叶的提取物进行分析, 筛选不同来源的桔梗茎叶差异代谢物。结果 桔梗茎叶中共检测到18个代谢产物, 其中包括黄酮类化合物4个, 炔苷类成分2个, 皂苷类成分12个(未见报道的代谢物1个), 并且推断出该未知化合物基本结构为远志酸型皂苷元-葡萄糖-木糖/阿拉伯糖苷。通过比较分析, 发现了不同来源的桔梗在化学成分上的差异, 筛选了6个中韩桔梗差异代谢物及1个延边地区桔梗差异代谢物。结论 桔梗茎叶具有丰富的化学成分, 不同来源的桔梗茎叶具有化合物差异性, 具有较高的开发利用前景, 为桔梗地上部资源的开发利用提供理论依据。

英文摘要:

      Objective To analyse the types and content of metabolites in the stems and leaves of Platycodon grandiflorus by ultra performance liquid chromatography-Quadrupole/Orbitrap high-resolution mass spectrometry (UPLC-Q/Orbitrap HRMS). Methods Stems and leaves of two-year-old purple flowered Platycodon grandiflorus from different sources was used as experimental materials. UPLC-Q/Orbitrap HRMS technology was used to analyze the extracts of Platycodon grandiflorus stems and leaves in negative ion mode, using orbitrap for MS1 full scan and data dependent acquisition ion trap for MS2 scan, and screen for differential metabolites of Platycodon grandiflorum stems and leaves from different sources. Results A total of 18 metabolites were detected in the stems and leaves of Platycodon grandiflorum, including 4 flavonoids, 2 alkynyl glycosides, and 12 saponins (1 metabolite that had not been reported). The basic structure of this unknown compound was polygalacic acid-glucose- xylose/arabic glycoside. Through comparative analysis, differences in chemical composition of Platycodon grandiflorus among different sources were discovered. The 6 differential metabolites of Platycodon grandiflorus from China and South Korea and 1 differential metabolite of Platycodon grandiflorus from Yanbian area were screened. Conclusion The comprehensive analysis results indicate that the stems and leaves of Platycodon grandiflorus have rich chemical components, and the stems and leaves of Platycodon grandiflorus from different sources have compound differences, which have high development and utilization prospects, providing theoretical basis for the development and utilization of aboveground resources of Platycodon grandiflorus.