三七须根提取液对小鼠酒精性肝损伤的保护作用研究

Protective effects of Panax notoginseng fibrous root extract on alcoholic liver disease in mice

中文关键词:  三七须根  酒精性肝损伤  氧化应激  小鼠

英文关键词:Panax notoginseng fibrous root  alcoholic liver disease  oxidative stress  mice

中文摘要:

      目的 探究三七须根提取液对酒精引起小鼠肝损伤的保护作用。方法 随机将50只雄性C57BL/6J小鼠分为对照组、模型组、低剂量组(67.5 mg/kg)、中剂量组(135.0 mg/kg)、高剂量组(270.0 mg/kg), 每组10只。对照组与模型组灌胃纯水, 三七须根提取液剂量组分别灌胃相应浓度的受试物20 mL/kg; 三七须根提取液剂量组与模型组5 h后灌胃40%乙醇10 mL/kg, 连续14 d, 最后一次灌胃后禁食16 h, 再分别给予20 mL/kg乙醇进行冲击。称量各组小鼠空腹体重后腹主动脉取血, 取肝脏称重, 后测定小鼠血清及肝匀浆生化指标以及白介素-1β (interleukin-1β, IL-1β)、白介素-6 (interleukin-6, IL-6)的水平。结果 三七须根提取液中、高剂量组与模型组相比, 血清中甘油三酯(triglyceride, TG)、谷丙转氨酶(alanine aminotransferase, ALT)、谷草转氨酶(aspartate aminotransferase, AST)显著下降(P<0.05或P<0.01); 肝脏中超氧化物歧化酶(superoxide dismutase, SOD)、谷胱甘肽(glutathione, GSH)、谷胱甘肽过氧化物酶(glutathione peroxidase, GSH-Px)的含量较模型组显著升高(P<0.05或P<0.01), 丙二醛(malondialdehyde, MDA)含量极显著下降(P<0.01); 高剂量组肝脏中TG含量与模型组相比, 差异具有极显著性(P<0.01); 低、高剂量组IL-1β水平与模型组相比, 显著降低(P<0.05或P<0.01)。结论 三七须根提取液对酒精诱导的小鼠肝损伤有较好的保护作用。

英文摘要:

      Objective To explore the protective effects of Panax notoginseng fibrous root extract on alcoholic liver disease in mice. Methods A total of 50 male C57BL/6J mice were randomly divided into 5 groups of 10 mice each: The control group, the model group, the low dose group (67.5 mg/kg), the medium dose group (135.0 mg/kg) and the high dose group (270.0 mg/kg). The control group and the model group were gavaged with pure water, while the Panax notoginseng fibrous root extract groups were given with the corresponding concentrations of the test substance at 20 mL/kg. The Panax notoginseng fibrous root extract groups and model group were gavaged with 40% ethanol (10 mL/kg) 5 hours late for 14 days. The Panax notoginseng fibrous root extract groups and model group were fasted for 16 hours after the last gavage, then each was given 40% ethanol at 20 mL/kg. After weighing the fasting body weight of each group of mice, blood was collected from the abdominal aorta and the liver was removed and weighed. Finally, determined the biochemical indices of serum and liver, and the levels of interleukin-1β (IL-1β) and interleukin-6 (IL-6) in liver. Results Compared with the model group, the medium and high dose groups of the serum triglyceride (TG), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were significantly decreased (P<0.05 or P<0.01). But the superoxide dismutase (SOD), glutathione (GSH) and glutathione peroxidase (GSH-Px) in liver was significantly increased (P<0.05 or P<0.01), while the content of malondialdehyde (MDA) was extremely significantly decreased (P<0.01). The content of TG in liver of high dose group was lower than the model group (P<0.01), the difference was statistical significance. Besides, the levels of IL-1β of the low and high dose groups were significantly decreased compared with the model group (P<0.05 or P<0.01). Conclusion The Panax notoginseng fibrous root extract has a good protective effect on hepatic injury induced by alcohol in mice.