| 刘小芳,李向阳,付晓艳,李福后,王伟霞,冷凯良.太平洋褶柔鱼内脏蛋白肽的抗氧化活性及稳定性研究[J].食品安全质量检测学报,2025,16(10):302-308 |
| 太平洋褶柔鱼内脏蛋白肽的抗氧化活性及稳定性研究 |
| Study on the antioxidant activity and stability of peptides from the visceral of Todarodes pacificus |
| 投稿时间：2024-12-04 修订日期：2025-04-20 |
| DOI： |
| 中文关键词: 太平洋褶柔鱼内脏 蛋白肽 抗氧化活性 稳定性 |
| 英文关键词:Todarodes pacificus visceral peptides antioxidant activity stability |
| 基金项目:青岛市关键技术攻关及产业化示范类项目(23-1-3-hysf-1-hy) |
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| Author | Institution |
| LIU Xiao-Fang | Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of Sustainable Development of Polar Fisheries,Ministry of Agriculture and Rural Affairs |
| LI Xiang-Yang | Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Polar Fisheries, Ministry of Agriculture and Rural Affairs；Jiangsu Ocean University, School of Ocean Food and Biological Engineering, Jiangsu Key Laboratory of Marine Biotechnology |
| FU Xiao-Yan | Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Polar Fisheries, Ministry of Agriculture and Rural Affairs;Jiangsu Ocean University, School of Ocean Food and Biological Engineering, Jiangsu Key Laboratory of Marine Biotechnology |
| LI Fu-Hou | Jiangsu Ocean University, School of Ocean Food and Biological Engineering, Jiangsu Key Laboratory of Marine Biotechnology |
| WANG Wei-Xia | Jiangsu Ocean University, School of Ocean Food and Biological Engineering, Jiangsu Key Laboratory of Marine Biotechnology |
| LENG Kai-Liang | Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Polar Fisheries, Ministry of Agriculture and Rural Affairs;Laboratory for Marine Drugs and Bioproducts, Qingdao Marine Science and Technology Center |
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| 中文摘要: |
| 目的 分析探究太平洋褶柔鱼（Todarodes pacificus，T. pacificus）内脏蛋白肽的抗氧化活性及稳定性。方法 通过系统测定太平洋褶柔鱼内脏蛋白肽的自由基清除能力，评价其抗氧化活性，并考察温度、酸碱、胃肠道消化等因素对其稳定性的影响。结果 太平洋褶柔鱼内脏蛋白肽具有良好的自由基清除能力，其清除1,1-二苯基-2-三硝基苯肼（1,1-Diphenyl-2-picrylhydrazyl radical，DPPH）自由基、2,2-联氮-2(3-乙基-苯并噻唑-6-磺酸)二铵盐[2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt，ABTS]阳离子自由基、羟自由基、超氧阴离子自由基的半数抑制浓度（half maximal inhibition concentration，IC50）分别为1.38、15.26、0.90、2.21 mg/mL。该蛋白肽的抗氧化活性在25~100 ℃温度范围内较为稳定；在pH 7.0~8.0条件下其抗氧化活性变化不显著（P＞0.05），但在强酸或强碱条件下其抗氧化活性显著下降（P＜0.05）；经体外模拟胃肠道消化后，该蛋白肽依然能够维持良好的抗氧化活性。结论 太平洋褶柔鱼内脏蛋白肽具备良好的抗氧化活性与稳定性，但在其加工与贮藏过程中还应注意避免高温与极端酸碱条件的影响。研究成果可为太平洋褶柔鱼内脏资源的高值化利用开辟新途径，也可为天然抗氧化肽的开发提供科学指导。 |
| 英文摘要: |
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