宏基因组测序分析熟制禽肉制品污染微生物中整合子组成

Analysis of integrative and conjugative elements composition in contaminated microorganisms of cooked poultry products by metagenomic sequencing

中文关键词:  熟制禽肉制品  宏基因组  整合子

英文关键词:cooked poultry meat products  metagenomics  integrative and conjugative elements

中文摘要:

      目的 研究熟制禽肉制品污染微生物中整合子的分布、来源及其携带的基因盒内容等相关情况。方法 采购熟制鸭肉, 对样品进行DNA提取, 测序、混合拼接、分箱, 获取宏基因组组装基因组, 整合子数据库比对。结果 熟制鸭肉污染微生物中检测到65种整合子, 共计110个, 主要来源于31种宿主微生物, 包括中生根瘤菌属、铜绿假单胞菌与肺炎克雷伯氏菌等; 整合子中共有9种转座酶, 1种毒力因子基因, 19个抗性相关基因, 其中四环素抗性基因10个, 防御基因11个, 均为防御噬菌体的基因。结论 多种整合子及毒力因子、抗性相关和防御基因的发现, 揭示了食品中微生物遗传多样性的复杂程度, 为研究基因的水平转移提供了重要线索, 对于评估食品安全性、预防和控制食源性病原体具有重要意义。

英文摘要:

      Objective To investigate the distribution, sources, and gene cassette contents of integrative and conjugative elements in microorganisms contaminating cooked poultry meat products. Methods Cooked duck meat was purchased, and DNA extraction, sequencing, assembly, binning, and metagenome-assembled genome (MAG) acquisition were performed. These MAGs were then compared with an integron database. Results A total of 110 integrative and conjugative elements belonging to 65 distinct types were detected in the contaminating microbiota of cooked duck meat, primarily originating from 31 host microorganisms, including Mesorhizobium, Pseudomonas aeruginosa, and Klebsiella pneumoniae. Among these integrative and conjugative elements, there were 9 kinds of transposases, 1 kinds of virulence factor gene, and 19 resistance-related genes, including 10 tetracycline resistance genes. Additionally, 11 defense genes, all of which were phage-defense genes, were identified. Conclusion The discovery of various integrative and conjugative elements, virulence factors, resistance-related genes, and defense genes reveals the complex genetic diversity of microorganisms in food. This provides important clues for studying horizontal gene transfer and has significant implications for assessing food safety and preventing and controlling foodborne pathogens. The effective application of metagenomic technology in integron research also demonstrates its potential in the fields of food safety and public health.