近红外光谱技术结合化学计量学快速检测蛋黄粉掺假

Rapid detection of egg yolk powder adulteration by Fourier transform near-infrared spectroscopy combined with chemometrics

中文关键词:  近红外光谱  化学计量学  蛋黄粉  掺假  快速

英文关键词:Fourier transform near-infrared spectroscopy  chemometrics  egg yolk powder  adulteration

中文摘要:

      目的 采用近红外光谱(Fourier transform near-infrared spectroscopy, FT-NIR)技术和化学计量学[无监督偏最小二乘判别分析(unsupervised partial least squares discriminant analysis, PLS-DA)]进行的蛋黄粉筛查指纹匹配定性方法, 用于在工厂接收环节检测蛋黄粉中潜在的食品欺诈问题。方法 通过向蛋黄粉样品中添加不同比例(质量百分比分别为5%、10%和25%)的淀粉和麦芽糊精、植脂末和大豆蛋白粉制备掺假样品, 使用FT-NIR检测收集真实样品和掺假样品的谱图, 并应用PLS-DA模型进行判别分析。结果 该方法经验证, 可用于检测蛋黄粉中淀粉和麦芽糊精、植脂末和大豆蛋白粉的含量, 质量百分比分别为5%、10%和25%。该方法不适用于含有少于5%上述潜在掺假成分的蛋黄粉。其他食品掺假成分的检出限值需要进行进一步验证。通过该方法分析的结果以“阴性”或“阳性”表示。结论 该方法适用于蛋黄粉掺假的快速检测, 可大大提高工厂接收环节检测蛋黄粉中潜在的食品欺诈效率。

英文摘要:

      Objective To detect potential food fraud issues in egg yolk powder during factory reception by a fingerprint matching method for egg yolk powder screening was developed using Fourier transform near-infrared spectroscopy (FT-NIR) technology and chemometrics [unsupervised partial least squares discriminant analysis (PLS-DA)]. Methods Adulterated samples were prepared by adding starch & maltodextrin, creamer, and soy protein fluor at different levels to egg yolk powder (5%, 10% and 25% by mass/mass). FT-NIR spectra for both authentic and adulterated samples were collected, and PLS-DA models were applied for discriminant analysis. Results The method was validated for detecting the levels of starch & maltodextrin, creamer, and soy protein flour in egg yolk powder at 5%, 10%, and 25% by mass/mass. The method was not applicable for egg yolk powder containing less than 5% of abovementioned adulterants. The limits of detection for other food adulterants needed further validation. The results analyzed by this method were expressed as “negative” or “positive”. Conclusion This method is suitable for rapid detection of counterfeit egg yolk powder and can greatly improve the efficiency of detecting potential food fraud in egg yolk powder during the factory receiving process.