应用胶体金免疫层析法检测肉类中多种动物源性成分

Application of colloidal gold immunochromatography for detecting various animal derived components in meat

中文关键词:  快速检测  动物源成分  实时荧光定量PCR  定性分析

英文关键词:colloidal gold immunochromatography  rapid detection  animal derived components  quantitative real-time polymerase chain reaction

中文摘要:

      目的 研究胶体金免疫层析法检测肉制品中动物源性成分的准确性及可行性。方法 利用该快速检测试剂盒检测肉制品中动物源性成分, 对样品中8种动物源成分的灵敏度、特异性、掺假模拟、假阳性率、假阴性率进行相关实验, 并以实时荧光定量聚合酶链式反应(quantitative real-time polymerase chain reaction, QPCR)为参比对照。结果 该快速检测试剂盒的灵敏度对牛、鸡、鸭、鹅的检出限可达到0.1%, 对猪、羊、马、驴的检出限可达到0.01%; 掺假模拟中猪、牛、羊、鸡、鸭、鹅、马、驴的检出限均可达到0.5%, 同时与其他非本物种通道检测时均无交叉反应, 特异性较好。结论 本研究建立的胶体金免疫层析法适用于肉制品中动物源性的现场快速筛查和检测。

英文摘要:

      Objective To study the accuracy and feasibility of colloidal gold immunochromatography for the detection of animal-derived components in meat products. Methods The rapid detection kit was used to detect the animal-derived components in meat products. The sensitivity, specificity, adulteration simulation, false positive rate and false negative rate of 8 kinds of animal-derived components were tested, and quantitative real-time polymerase chain reaction (QPCR) was used as a reference. Results The limits of detection of the rapid detection kit could reach 0.1% for cattle, chicken, duck and goose, and 0.01% for pig, sheep, horse and donkey. The limits of detection of pig, cattle, sheep, chicken, duck, goose, horse and donkey in the adulterated simulation could reach 0.5%, and there was no cross-reaction with other non-species channel detection, and the specificity was good. Conclusion The colloidal gold immunochromatography method established in this study is suitable for the rapid screening and detection of animal origin in meat products.