QuEChERS dSPE EMR-Lipid-液相色谱-四极杆飞行时间质谱法定性筛查粮谷中29种真菌毒素

Qualitative screening of 29 kinds of mycotoxins in grain by QuEChERS dSPE EMR-Lipid and liquid chromatography-quadrupole time of flight-mass spectrometry

中文关键词:  液相色谱-四极杆/飞行时间质谱  QuEChERS  真菌毒素  粮谷

英文关键词:liquid chromatography-quadrupole time of flight-mass spectrometry  QuEChERS  mycotoxins  grain  qualitative screening

中文摘要:

      目的 建立QuEChERS dSPE EMR-Lipid结合液相色谱-四极杆飞行时间质谱法定性筛查粮谷中29种真菌毒素残留的方法。方法 样品经20 mL 70%乙腈(含1%甲酸)水溶液提取, 改良QuEChERS法净化, 电喷雾电离, 正离子扫猫, 顺序窗口采集所有理论质谱模式。结果 建立了包含一级精确质量、二级质谱和保留时间的数据库, 该方法实现了黄大豆、玉米、大米和小麦中29种真菌毒素的快速筛查, 筛查检出限分布在0.005~0.050 mg/kg之间, 均能满足筛查基本要求。结论 该方法操作简便、特异性好、稳定可靠, 较短时间内完成黄大豆、玉米、大米和小麦中大批量样品的处理及定性筛查, 具有较高的应用价值。

英文摘要:

      Objective To establish a qualitative screening method for 29 kinds of mycotoxins in grains based on QuEChERS dSPE EMR-Lipid and liquid chromatography-quadrupole time of flight-mass spectrometry. Methods The sample was extracted by 20 mL 70% acetonitrile (containing 1% formic acid), purified by modified QuEChERS method, electrospray ionization, positive ion sweeping, sequential window acquisition of all theoretical mass spectra. Results The established method included a database of primary precision mass, secondary mass spectrometry, and retention time. This method enabled rapid screening of 29 kinds of mycotoxins in soybean, corn, rice, and wheat. The screening limits of detection of ranged from 0.005 to 0.050 mg/kg. The method met the basic screening requirements. Conclusion The method is simple, specific, stable and reliable. The method enables the processing and qualitative screening of large volumes of samples from soyabean, corn, rice and wheat in a short time. It has high application value.