响应面法优化肉苁蓉多糖提取液的脱色工艺及其抗氧化活性研究

Optimization of decolorization process and antioxidant activity of Cistanche deserticola polysaccharide extract by response surface methodology

中文关键词:  肉苁蓉多糖  脱色  响应面法  抗氧化活性

英文关键词:Cistanche deserticola polysaccharide  decolorization  response surface methodology  antioxidant activity

中文摘要:

      目的 优化肉苁蓉多糖的脱色工艺并探究其抗氧化活性。方法 以活性炭为脱色剂, 分别考察了活性炭用量、脱色温度、脱色时间和pH 4个因素对脱色率和多糖回收率的影响, 在单因素实验的基础上, 利用响应面法优化肉苁蓉多糖提取液的脱色工艺。利用体外抗氧化活性评价法, 测定肉苁蓉多糖的1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazine, DPPH)自由基清除能力、总还原能力、羟自由基清除能力和2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐[2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, ABTS]阳离子自由基清除能力。结果 肉苁蓉多糖提取液最优脱色工艺条件为: 活性炭用量20%, 脱色温度37 ℃, 脱色时间49 min, pH 5.03, 在此条件下, 肉苁蓉多糖的脱色率为62.66%, 多糖回收率为96.16%。脱色后, 肉苁蓉多糖的DPPH自由基和羟自由基清除能力显著提高, 而ABTS阳离子自由基清除能力明显下降。结论 该脱色工艺操作简单, 脱色率和多糖回收率高, 得到的肉苁蓉多糖具有重要的应用价值, 为产品开发提供依据。

英文摘要:

      Objective To optimize the decolorization process of Cistanche deserticola polysaccharides and explore their antioxidant activity. Methods Using activated carbon as a decolorizing agent, the effects of 4 factors including activated carbon dosage, decolorization temperature, decolorization time, and pH on decolorization rate and polysaccharide recovery rate were investigated. Based on single factor experiments, the decolorization process of Cistanche deserticola polysaccharide extract was optimized using response surface methodology. Using an in vitro antioxidant activity evaluation method, the free radical scavenging ability of 1,1-diphenyl-2-picrylhydrazine (DPPH), total reducing ability, hydroxyl radical scavenging ability, and 2,2’-azino bis(3-ethylbenzothiazole- 6-sulfonic acid) diammonium salt (ABTS) cation free radical scavenging ability of the polysaccharides from Cistanche deserticola were determined. Results The optimal decolorization process conditions for the extract of polysaccharides from Cistanche deserticola were as follows: Active carbon dosage 20%, decolorization temperature 37 ℃, decolorization time 49 min, pH 5.03. Under these optimal conditions, the decoloizration rate and polysaccharide recovery rate were 62.66% and 96.16%, respectively. After decolorization, the capabilities of scavenging DPPH radical and hydroxyl free radical was significantly increased, while the capabilities of scavenging ABTS anion radical was significantly decreased. Conclusion The decolorization process is easy to operate, with good decolorization effects and polysaccharide recovery rate. It has important application value and provides basis for the later research and development of Cistanche deserticola polysaccharide.