新型低共熔溶剂对茶多糖吸光度的影响

Effects of new deep eutectic solvent on absorbance of tea polysaccharide

中文关键词:  低共熔溶剂  茶多糖  检测方法

英文关键词:deep eutectic solvents  tea polysaccharide  detection methods

中文摘要:

      目的 探究新型低共熔溶剂(deep eutectic solvent, DES)在可见分光光度法检测中对茶多糖吸光度的影响, 建立快速高效的茶多糖含量检测方法。方法 氯化胆碱/DL-酒石酸DES作为茶多糖的提取溶剂, 考察蒽酮-硫酸和苯酚-硫酸两种检测方法中DES对茶多糖含量检测的影响, 并对方法学线性关系、精密度、稳定性、重复性和加标回收率进行比较。结果 茶多糖测定的最佳方法为苯酚-硫酸法, 最适检测波长为484 nm, 葡萄糖质量浓度在20~100 μg/mL之间吸光度具有良好的线性关系, 其精密度相对标准偏差(relative standard deviation, RSD)为0.10%, 稳定性RSD为0.00%, 重复性RSD为2.40%, 加标回收率为94.25%~111.19%, 测得茶多糖含量为54.76 mg/g。结论 苯酚-硫酸法适用于以DES为提取溶剂的茶叶中茶多糖含量测定。

英文摘要:

      Objective To study the effects of deep eutectic solvent (DES) on the absorbance of tea polysaccharide by visible spectrophotometry, and establish a fast and efficient method for the determination of tea polysaccharide. Methods Choline chloride/DL-tartaric acid was used as the extraction solvent of tea polysaccharide. The influence of DES on the determination of tea polysaccharide content in anthrone sulfuric acid and phenol-sulfuric acid 2 kinds of detection methods was investigated. The linear relationship, precision, stability, repeatability and standard recovery rate of the 2 kinds of detection methods were compared. Results The best method for the determination of tea polysaccharides was phenol-sulfuric acid method, the optimal detection wavelength was 484 nm, and the absorbance had a good linear relationship with glucose concentration between 20 and 100 μg/mL. The precision relative standard deviation (RSD) of this method was 0.10%, the stability RSD was 0.00%, the repeatability RSD was 2.40%, and the recovery rate was 94.25%–111.19%, and the content of tea polysaccharide was 54.76 mg/g. Conclusion Phenol-sulfuric acid method is suitable for the determination of tea polysaccharide in tea with DES as extraction solvent.