基于纳米银修饰氧化锰纳米酶评价扬州市野生食用菌的总抗氧化能力

Evaluation of total antioxidant capacity of wild edible mushrooms in Yangzhou based on nano silver modified manganous oxide nanozyme

中文关键词:  野生食用菌  总抗氧化活性  Mn/Ag NPs纳米酶  比色反应  便捷评价

英文关键词:wild edible mushrooms  total antioxidant capacity  nano silver modified manganous oxide nanozyme  colorimetric reaction  convenient evaluation

中文摘要:

      目的 开发一种基于纳米酶的比色分析方法, 用于扬州市野生食用菌(角质木耳、金针菇、糙皮侧耳)总抗氧化能力的快速评价。方法 利用水热法制备了纳米银修饰氧化锰(nano silver modified manganous oxide, Mn/Ag NPs)纳米酶, 并通过多种表征手段确认其微观结构。利用不同溶剂提取3种野生食用菌的活性物质, 并通过基于Mn/Ag NPs纳米酶的比色分析方法评价扬州市3种野生食用菌的抗氧化活性。此外, 利用商业化2,2’-联氮-双(3-乙基苯并噻唑啉-6-磺酸)[2,2’-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid), ABTS]试剂盒对评价结果进行验证。结果 基于Mn/Ag NPs纳米酶的比色分析方法实现了扬州市野生食用菌总抗氧化能力的快速评价。总抗氧化活性: 糙皮侧耳>金针菇>角质木耳; 溶剂提取效果: 超纯水>混合溶剂>乙醇>乙酸乙酯。结论 基于Mn/Ag NPs纳米酶的比色分析方法能够快速评价扬州市野生食用菌的抗氧化活性。

英文摘要:

      Objective To develop a colorimetric analysis method based on nanozymes, rapidly evaluate the total antioxidant capacity of wild edible mushrooms (Auricularia cornea, Flammulina filiformis, and Pleurotus ostreatus) in Yangzhou. Methods Nano silver modified manganous oxide (Mn/Ag NPs) nanozyme was prepared by the hydrothermal method, and its microstructure was confirmed by various characterization methods. The total antioxidant capacity of 3 kinds of wild edible mushrooms were extracted using different solvents, and their total antioxidant capacity were evaluated by the colorimetric analysis method based on Mn/Ag NPs nanozyme. In addition, the evaluation results were verified using the commercial 2,2’-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) kit. Results The colorimetric analysis method based on Mn/Ag NPs nanozyme achieved the rapid evaluation of the total antioxidant capacity of wild edible mushrooms in Yangzhou. Total antioxidant activity was Pleurotus ostreatus>Flammulina filiformis>Auricularia cornea. Extraction efficiency of solvent was ultrapure water>mixed solvent>ethanol>ethyl acetate. Conclusion The colorimetric analysis method based on Mn/Ag NPs nanozyme can rapidly evaluate the total antioxidant capacity of wild edible mushrooms in Yangzhou.