胶体金免疫层析法快速检测蔬菜中的啶虫脒含量

Rapid detection of acetamiprid in vegetables using colloidal gold immunochromatography assay

中文关键词:  啶虫脒  单克隆抗体  胶体金试纸条  呈色分析仪  定量检测

英文关键词:acetamiprid  monoclonal antibody  gold immunochromatography assay  colorimetric analyzer  quantitative determination

中文摘要:

      目的 制备胶体金免疫层析法试纸条, 实现蔬菜中啶虫脒残留量的快速检测。方法 通过半抗原合成获得免疫抗原, 通过动物免疫和杂交瘤技术获得啶虫脒高灵敏特异性单克隆抗体, 基于该抗体优化划膜条件等参数确定, 制备了免疫胶体金试纸条, 该试纸条联合呈色分析应用于多种蔬菜的定量检测。结果 在最适工作条件下, 所建立的方法对啶虫脒的检出限为0.23 μg/kg, 线性范围为0.42~18.38 μg/kg。实际样品检测回收率为70.0%~88.3%, 测定批内和批间实验的变异系数(coefficient of variation, CV)分别低于12.00%和11.03%。结论 该快速检测试纸条可直接应用于蔬菜中啶虫脒的现场高通量快速筛查, 借助呈色分析仪亦可实现对啶虫脒的定量检测。

英文摘要:

      Objective To prepare colloidal gold immunochromatographic test strips for the rapid detection of acetamiprid residues in vegetables. Methods The immunogen was obtained through hapten synthesis, and a highly sensitive and specific monoclonal antibody against acetamiprid was developed using animal immunization and hybridoma technology. Based on this antibody, parameters such as membrane-coating conditions were optimized to prepare immunocolloidal gold test strips. These test strips, combined with colorimetric analysis, were applied for the quantitative detection of acetamiprid residues in various vegetables. Results Under optimal working conditions, the established method achieved a limit of detection of 0.23 μg/kg for acetamiprid, with a linear range of 0.42–18.38 μg/kg. The recovery rates for actual sample detection ranged from 70.0% to 88.3%, and the coefficients of variation (CV) for intra-batch and inter-batch experiments were below 12.00% and 11.03%, respectively. Conclusion The rapid test strips can be directly applied for high-throughput on-site screening of acetamiprid residues in vegetables. Additionally, with the assistance of a colorimetric analyzer, quantitative acetamiprid detection can also be achieved.