大体积进样-离子色谱法测定食品接触用塑料制品中高氯酸盐迁移量

Determination of perchlorate migration in food contact plastic products by large volume injection-ion chromatography

中文关键词:  高氯酸盐  离子色谱法  食品接触用塑料  迁移量

英文关键词:perchlorate  large volume injection-ion chromatography  food contact plastics  migration

中文摘要:

      目的 建立并优化大体积进样-离子色谱法(ion chromatography, IC)测定食品接触用塑料制品中高氯酸盐(ClO4-)迁移量的检测方法。方法 选用水、3%乙酸(m:V)、4%乙酸(V:V)、10%乙醇(V:V)、20%乙醇(V:V)、50%乙醇(V:V)、95%乙醇(V:V)作为食品模拟物, 以14.0 mmol/L无水碳酸钠和20%乙腈(体积分数)混合溶液作为流动相进行等度洗脱, 进样量800 μL, 流速1.0 mL/min, 采用Metrosep A Supp 4离子色谱柱(250 mm×4.0 mm, 9.0 μm)和Metrosep A Supp RP Guard保护柱(50 mm×4.0 mm, 9.0 μm), 柱温30 ℃, 以电导检测器进行测定, 外标法定量。结果 方法验证结果表明, 该方法线性关系良好, 标准工作曲线的线性相关系数大于0.995, 方法检出限为0.15 μg/kg, 定量限为0.50 μg/kg, 加标回收率为83.2%~99.1%, 精密度为2.7%~9.1% (n=6)。结论 该方法可靠、方便、灵敏, 分离效果好, 适用于食品接触用塑料制品中高氯酸盐迁移量的检测。

英文摘要:

      Objective To establish and optimize a method for the determination of perchlorate (ClO4-) migration in food contact plastic products by large volume injection-ion chromatography (IC). Methods Water, 3% acetic acid (m:V), 4% acetic acid (V:V), 10% ethanol (V:V), 20% ethanol (V:V), 50% ethanol (V:V), and 95% ethanol (V:V) were selected as food simulants, and the isocratic elution was performed with a mixture of 14 mmol/L anhydrous sodium carbonate and 20% acetonitrile (volume fraction) as mobile phases, with an injection volume of 800 μL and a flow rate of 1.0 mL/min. The separation was performed on a Metrosep A Supp 4 ion chromatography column (250 mm× 4.0 mm, 9.0 μm) and a Metrosep A Supp RP Guard guard column (50 mm×4.0 mm, 9.0 μm) at a column temperature of 30 ℃ with a conductivity detector for the determination and the quantification of the extract by the external standard method. Results The method validation results showed that the method was linear with the linear correlation coefficient of the standard working curve greater than 0.995. The limit of detection was 0.15 μg/kg, the limit of quantification was 0.50 μg/kg, and the recoveries were 83.2%~99.1%, and the precision was 2.7%~9.1% (n=6). Conclusion The method is reliable, convenient, sensitive, has good separation effects, and suitable for the determination of perchlorate migration in food contact plastic products.