三磷酸腺苷生物发光法在冷鲜肉微生物检测中的应用研究

Application of adenosine triphosphate bioluminescence method in the microbial detection of chilled meat

中文关键词:  冷鲜肉  菌落总数  平板计数法  ATP生物发光法

英文关键词:chilled meat  colonies number  plate count method  adenosine triphosphate bioluminescence method

中文摘要:

      目的 探究三磷酸腺苷(adenosine triphosphate, ATP)生物发光法检测冷鲜肉中菌落总数的可行性, 比较其与国家标准平板计数法的检测效果。方法 通过实验确定不同浓度的曲拉通X-100 (Triton X-100)和三磷酸腺苷双磷酸酶(adenosine triphosphate double, Apyrase)对冷鲜肉体细胞ATP的清除效果, 并分别使用ATP生物发光法和国家标准平板计数法检测冷鲜肉中的菌落总数, 并评估ATP生物发光法的重现性。结果 Triton X-100和Apyrase清除冷鲜肉体细胞ATP的最佳浓度分别是0.2%和0.10 U/mL, 此浓度下, 冷鲜肉体细胞ATP清除效果最好。ATP生物发光法与国家标准平板计数法的检测结果显示出良好的线性关系, 其相关系数(r2)为0.9871, 且ATP生物发光法重现性良好, 检测结果稳定可靠。结论 本研究通过与国家标准平板计数法的比较, 进一步验证了ATP生物发光法可用于冷鲜肉中菌落总数的快速检测。

英文摘要:

      Objective To investigate the feasibility of using the adenosine triphosphate (ATP) bioluminescence method for detecting the colonies number in chilled meat, and compare its performance with the national standard plate count method. Methods Effects of different concentrations of Triton X-100 and adenosine triphosphate double (Apyrase) on the remove of ATP from the somatic cells of chilled meat were determined. The colonies number in the chilled meat was measured using both the ATP bioluminescence method and the national standard plate count method, and the reproducibility of the ATP bioluminescence method was evaluated. Results The optimal concentrations of Triton X-100 and Apyrase for ATP removal from the somatic cells of chilled meat were found to be 0.2% and 0.10 U/mL, respectively, with the best ATP removal effect observed at these concentrations. The results of the ATP bioluminescence method and the national standard plate count method demonstrated a strong linear relationship, with a correlation coefficient (r2) of 0.9871. The ATP bioluminescence method indicated good reproducibility, and the results were stable and reliable. Conclusion This study further validates the applicability of ATP bioluminescence method for the quick detection of colonies number in chilled meat, demonstrating its advantages over the national standard plate count method.