柳州螺蛳粉生产链中副溶血性弧菌的污染状况研究

Study on the contamination status of Vibrio parahaemolyticus in the production chain of Liuzhou river snails rice noodles

中文关键词:  预包装柳州螺蛳粉  生产链  副溶血性弧菌  污染状况

英文关键词:Liuzhou river snails rice noodles  production chain  Vibrio parahaemolyticus  contamination status

中文摘要:

      目的 调查预包装柳州螺蛳粉生产链中副溶血性弧菌的污染水平, 及时了解揭示生产链中污染副溶血性弧菌(Vibrio parahaemolyticus, VP)风险大小。方法 2023—2024年采用随机的方式收集柳州市农贸市场和柳州螺蛳粉生产企业的螺蛳及螺肉、半成品汤料包和预包装螺蛳粉终产品共487份, 利用培养法结合实时荧光聚合酶链式反应(polymerase chain reaction, PCR)法对VP进行精准检测。结果 农贸市场的70份螺蛳中VP的检出率为38.6%, 对其中的44份进行定量检测, 平均污染量为16.1 MPN/g; 生产企业的41份螺蛳及螺肉中VP的检出率为2.4%; 半成品汤料包和终产品中均未检出VP。结论 尽管零售端螺蛳中VP污染较为普遍, 但在生产端的螺蛳及螺肉中VP污染率较低。此外, 螺蛳粉生产工艺中的熬煮过程也能有效杀灭VP。总体而言, 螺蛳粉生产链中污染VP的风险较小。

英文摘要:

      Objective To investigate the contamination level of Vibrio parahaemolyticus (VP) in the production chain of the Liuzhou river snails rice noodles, promptly assess the risk of VP in the production chain. Methods From 2023 to 2024, a total of 487 simlpes, including snails and snail meat, semi-finished soup packets, and pre-packed Liuzhou river snails rice noodles final products, were randomly collected from Liuzhou agricultural markets and manufacturing enterprises in Liuzhou. A conbination of culture-based methods and real-time fluorescent polymerase chain reaction (PCR) was used to detect VP. Results Among 70 snails samples from agricultural market with a detection rate of 38.6% and an average contamination level of 16.1 MPN/g in 44 snails samples. In the 41 snails and snail meat sample from manufacturing enterprises showing the detection rate of 2.4%; no VP was detected in the semi-finished soup package or final product. Conclusion Although VP contamination is relatively common in snails at the retail trade, the contamination rate of snails and snail meat in the enterprise is low. Furthermore, the repeated boiling in Liuzhou river snails rice noodles production can effectively control VP. Therefore, the risk of VP contamination in the Liuzhou river snails rice noodles production chain is relatively low.