刺参自溶肽美拉德反应产物表征及抗氧化活性研究

Characterization and antioxidant activity of Maillard reaction products of autolytic peptides from Stichopus japonicus

中文关键词:  刺参、自溶肽、美拉德反应、抗氧化活性

英文关键词:Stichopus japonicus  autolytic peptides  Maillard reaction  antioxidant activity

中文摘要:

      目的 探究刺参自溶肽美拉德反应产物的表征及抗氧化活性变化。方法 利用刺参自溶来制备自溶肽, 进一步采用D-木糖和刺参自溶肽的美拉德反应对其进行改性, 分析美拉德反应产物的理化性质和抗氧化活性差异。结果 选择美拉德反应条件为: 反应时间4 h, 反应温度120 ℃, 刺参自溶肽与D-木糖的质量比为1:2。反应后游离氨基含量由(32.35±1.90) μg/mL下降至(12.68±1.10) μg/mL, 反应前后荧光发射光谱最大吸收峰由401 nm移到434 nm, 紫外吸收光谱在288 nm处出现吸收峰, 分子量测定发现3600 u以上大分子量物质显著增加, 验证了刺参自溶肽与D-木糖美拉德反应产物的出现。美拉德反应产物在质量浓度为8 mg/mL时的羟自由基、1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazine, DPPH)自由基、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐[2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, ABTS]阳离子自由基清除能力均在85%以上, Fe3+还原能力比原刺参自溶肽提高6.3倍。结论 研究结果为利用刺参自溶现象, 改善刺参自溶肽抗氧化活性提供了新参考。

英文摘要:

      Objective To study the characterization and antioxidant activity of Maillard reaction products of autolytic peptides from Stichopus japonicus. Methods The autolysis phenomenon of Stichopus japonicus was taken to dissolve their body walls. Further used D-xylose and Stichopus japonicus autolytic peptides for Maillard reaction to modify autolytic peptides, and the physical and chemical properties and antioxidant activity differences of the Maillard reaction products were analyzed. Results The Maillard reaction conditions were as follows: Reaction time 4 h, reaction temperature 120 ℃, mass ratio of Stichopus japonicus autolytic peptides to D-xylose 1:2. After the reaction, the content of free amino group decreased from (32.35±1.90) μg/mL to (12.68±1.10) μg/mL. The maximum fluorescence emission peak of the Maillard reaction products shifted from 401 nm to 434 nm, and an absorption peak appeared at 288 nm in the ultraviolet absorption spectrum. The molecular weight measurement showed that the large molecular weight material above 3600 u increased significantly. The appearance of the Maillard reaction products from the autolytic peptides of Stichopus japonicus and D-xylose was verified. When the mass concentration of Maillard reaction products was 8 mg/mL, the scavenging ability of hydroxyl free radical, 1,1-diphenyl-2-picrylhydrazine (DPPH) free radical and 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) cation free radical were above 85%, and the reducing ability of Fe3+ was 6.3 times higher than that of autolytic peptides from Stichopus japonicus before the reaction. Conclusion The results provide a new reference for utilizing the autolysis of Stichopus japonicus and improving the antioxidant activity of the Stichopus japonicus autolytic peptides.