余甘子枸杞复方改善小鼠睡眠功能研究

Study on the sleep improvement function of Phyllanthus emblica L. and Lyciumbarbarum L. compound in mice

中文关键词:  余甘子枸杞复方  余甘子  改善睡眠  机制

英文关键词:Phyllanthus emblica L. and Lyciumbarbarum L.compound  sleep improvement  mice sleep model

中文摘要:

      目的 评估余甘子枸杞复方对小鼠改善睡眠的影响。方法 采用Balb-c小鼠作为研究对象, 随机将其分为5组: 空白组、阳性药组、余甘子枸杞复方低剂量组(3.75 g/kg)、中剂量组(7.50 g/kg)和高剂量组(15.00 g/kg)。每组小鼠每日接受一次给药, 持续30 d。通过实施直接睡眠实验、戊巴比妥钠阈下剂量催眠实验、戊巴比妥钠睡眠时间延长实验以及巴比妥钠睡眠潜伏期实验, 对余甘子枸杞复方改善小鼠睡眠的功效进行了评估。此外, 采用酶联免疫分析试剂盒比色法检测小鼠脑组织中血清素5-羟色胺(5-hydroxytryptamine, 5-HT)、多巴胺(dopamine, DA)、色氨酸羟化酶(tryptophan hydroxylase, TPH)以及γ-氨基丁酸(γ-aminobutyric acid, GABA)的水平。结果 与空白组相比, 余甘子枸杞复方的3个剂量组分别使小鼠睡眠率提高了50%、70%和90%。同时, 3个剂量组均显著延长了小鼠戊巴比妥钠诱导的睡眠时间(P<0.01), 且高剂量组的小鼠睡眠时间延长显著高于阳性药组(P<0.01)。此外, 中、高剂量组余甘子枸杞复方能显著缩短小鼠的睡眠潜伏期(P<0.05或P<0.01)。神经递质指标测定结果显示, 与空白对照组相比, 余甘子枸杞复方能显著提高小鼠脑组织中5-HT和GABA的含量(P<0.01或P<0.05), 并显著降低DA的含量(P<0.01); 但对小鼠脑组织中TPH含量无显著影响(P>0.05)。结论 余甘子枸杞复方对小鼠睡眠具有显著的改善作用。

英文摘要:

      Objective To evaluate the effect of Phyllanthus emblica L. and Lyciumbarbarum L. compound on sleep improvement in mice. Methods Balb-c mice were used as the research subjects and randomly divided into 5 groups: Control group, positive drug group, low-dose group of Phyllanthus emblica L. and Lyciumbarbarum L. compound (3.75 g/kg), medium-dose group (7.50 g/kg), and high-dose group (15.00 g/kg). Each mice received a single dose daily for 30 consecutive days. The efficacy in improving sleep in mice was assessed through direct sleep experiments, pentobarbital sodium subthreshold dose hypnosis experiments, pentobarbital sodium sleep time extension experiments, and barbiturate sodium sleep latency experiments. Additionally, the levels of 5-hydroxytryptamine (5-HT), dopamine (DA), tryptophan hydroxylase (TPH), and γ-aminobutyric acid (GABA) in the brain tissue of mice were measured using enzyme-linked immunosorbent assay colorimetric kits. Results The results showed that compared with the control group, the 3 dose groups of the combination of Phyllanthus emblica L. and Lyciumbarbarum L. compound increased the sleep rate of mice by 50%, 70%, and 90%, respectively. Additionally, all 3 dose groups significantly prolonged the pentobarbital sodium-induced sleep time in mice (P<0.01), and the high-dose group had a significantly longer sleep time than the positive drug group (P<0.01). Furthermore, the medium and high-dose groups of Phyllanthus emblica L. and Lyciumbarbarum L. compound significantly shortened the sleep latency of mice (P<0.05 or P<0.01). The measurement of neurotransmitter indicators showed that compared with the control group, the Phyllanthus emblica L. and Lyciumbarbarum L. compound, significantly increased the levels of 5-HT and GABA in the brain tissue of mice (P<0.01 or P<0.05) and significantly reduced the content of DA (P<0.01). However, it had no significant effect on the TPH content in the brain tissue of mice (P>0.05). Conclusion The Phyllanthus emblica L. and Lyciumbarbarum L. compound has a significant effect on improving sleep in mice.