不同品种龙眼核多酚的提取、抗氧化和抑菌活性的研究

Study on the extraction, antioxidant and antibacterial activities of polyphenols from different varieties of longan seeds

中文关键词:  龙眼核  多酚  品种  抗氧化  抑菌

英文关键词:longan seed  polyphenol  varieties  antioxidant  antibacterial

中文摘要:

      以不同品种的龙眼核样品为研究对象(分别来自储良龙眼，大广眼，双孖木龙眼，水涨龙眼，凤梨穗龙眼，泰国龙眼，石峡龙眼，福眼8个品种)，经过预处理后提取纯化的龙眼核多酚，采用 2, 2 - 联苯基 -1- 苦基肼基 ( 2 ,2 - Diphenyl -1- picrylhydrazyl，DPPH ) 自由基清除能力测试、2,2"" - 氨基苯并三唑- 6 -磺酸 ( 2,2"" - azinobis ( 3 -ethylbenzothiazoline - 6 - sulfonic acid, ABTS )以及抑菌圈实验对其抗氧化和抗菌功能进行分析，比较不同品种之间的差异。结果表明， 各品种龙眼核多酚均表现出较强的抗氧化能力，DPPH及ABTS清除率最高分别可达 98.70% 和 91.88%。龙眼核多酚对金黄色葡萄球菌、李斯特菌和枯草芽孢杆菌的生长均具有显著的抑制效果，抑菌圈直径在 16～24mm 。相关性分析结果显示，8个品种龙眼核多酚具有良好的抗氧化能力；双孖木龙眼和石峡龙眼对金黄色葡萄球菌有抑制作用，泰国龙眼对枯草芽孢杆菌有较强对抑制作用，储良龙眼对李斯特菌有强抑制作用。该实验为不同品种的龙眼核多酚的生物活性提供了参考依据。

英文摘要:

      This study investigated the polyphenols extracted and purified from various longan seed cultivars, specifically the following eight varieties: Chuliang, Daguan, Shuangzimu, Shuizhang, Fengli Sui, Thai, Shixia, and Fuyan longans. After pretreatment, the antioxidant and antibacterial properties of the longan seed polyphenols were analyzed using the DPPH (2,2-Diphenyl-1-picrylhydrazyl) radical scavenging assay, the ABTS (2,2""-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)) assay, and inhibition experiments. The results indicate that polyphenols from all longan seed varieties exhibited substantial antioxidant activity, with DPPH and ABTS scavenging rates reaching 98.70% and 91.88%, respectively. Moreover, these polyphenols effectively inhibited the growth of Staphylococcus aureus, Listeria monocytogenes, and Bacillus subtilis, with inhibition zone diameters ranging from 16 to 24 mm. Correlation analysis revealed that the eight longan seed polyphenols possess significant antioxidant capabilities; specifically, Shuangzimu and Shixia longan demonstrated inhibitory effects against Staphylococcus aureus, while Thai longan showed strong inhibition against Bacillus subtilis, and Shuliang longan effectively inhibited Listeria monocytogenes. This study provides a valuable reference for the bioactivity of polyphenols derived from different longan seed varieties.