白花木瓜多酚提取工艺优化及其抗氧化活性研究

Optimization of extraction process and study on antioxidant activity of Chaenomeles sinensis polyphenols

中文关键词:  白花木瓜  多酚  超声辅助提取  工艺优化  抗氧化活性

英文关键词:Chaenomeles sinensis  polyphenols  ultrasonic-assisted extraction  process optimization  antioxidant activity

中文摘要:

      目的 优化白花木瓜多酚提取工艺并研究其抗氧化活性。方法 通过单因素和正交实验对白花木瓜多酚的提取工艺进行优化; 同时开展体外实验, 对白花木瓜多酚的总还原能力、1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl, DPPH)自由基清除能力和羟基自由基清除能力进行研究。结果 白花木瓜多酚提取的最佳工艺为: 乙醇体积分数60%、料液比1:25 (g/mL)、超声温度55 ℃、超声时间90 min, 此条件下多酚提取量为2.22 mg/g; 在样品质量浓度为1.0 mg/mL时, 测定白花木瓜多酚总还原能力、DPPH自由基清除率和羟自由基清除率, 分别为2.41±0.02、89.33%±0.24%、81.30%±1.27%, 均高于同等浓度下的维生素C (2.29±0.08、86.05%±0.39%、80.94%±0.41%), 说明白花木瓜多酚具有较好的抗氧化活性, 有较好的应用前景。结论 本研究为白花木瓜多酚的进一步研究应用提供参考, 为白花木瓜资源多元高值化利用提供新思路, 为白花木瓜资源的深加工以及合理选择膳食提供了理论依据。

英文摘要:

      Objective To optimize the extraction process of Chaenomeles sinensis polyphenols and study their antioxidant activity. Methods The extraction process of Chaenomeles sinensis polyphenols was optimized through single factor and orthogonal experiments; the in vitro experiments were simultaneously conducted to investigate the total reducing ability, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging ability, and hydroxyl radical scavenging ability of Chaenomeles sinensis polyphenols. Results The optimal process for extracting polyphenols from Chaenomeles sinensis was: Ethanol volume fraction of 60%, solid-liquid ratio of 1:25 (g/mL), ultrasonic temperature of 55 ℃ and ultrasonic time of 90 min. Under these conditions, the polyphenol extraction amount was 2.22 mg/g; at a sample concentration of 1.0 mg/mL, the total reducing ability, DPPH radical scavenging rate and hydroxyl radical scavenging rate of Chaenomeles sinensis polyphenols were determined to be 2.41±0.02, 89.33%±0.24%, and 81.30%±1.27%, respectively, all higher than vitamin C at the same concentration (2.29±0.08, 86.05%±0.39%, 80.94%±0.41%), indicating that Chaenomeles sinensis polyphenols had good antioxidant activity and promising application prospects. Conclusion This study provides a reference for further research and application of polyphenols in Chaenomeles sinensis, offers new ideas for the diversified and high-value utilization of Chaenomeles sinensis resources, and provides theoretical basis for the deep processing of Chaenomeles sinensis resources and rational dietary selection.