环介导等温扩增技术在真菌检测中的应用研究进展

Research progress on the application of loop-mediated isothermal amplification in the detection of fungi

中文关键词:  环介导等温扩增技术  真菌  核酸扩增

英文关键词:loop-mediated isothermal amplification technique  fungi  nucleic acid amplification

中文摘要:

      真菌是日常生活中较为常见的一种微生物, 与我们的生活息息相关、有利有弊。其有害影响体现在多个方面, 例如有些真菌会导致农作物病害而减产, 误食毒蕈会引起食物中毒, 威胁人们的生命安全, 此外致病真菌还会引起真菌感染等疾病危害人们的身体健康, 因此能快速、准确地检测出病原真菌和识别出毒蕈是非常重要的。环介导等温扩增技术(loop-mediated isothermal amplification, LAMP)是近几年广泛使用的一种核酸扩增技术, 该技术使用DNA聚合酶和特异性引物, 在恒温条件下, 短时间内高效率扩增核酸, 相较于传统的分子生物学方法, 该方法对仪器要求不高, 具有快速、灵敏、简单等特点。目前LAMP已广泛应用于细菌、真菌和病毒等微生物的检测, 本文对LAMP在真菌检测方面的应用进行总结, 以期为相关研究提供参考。

英文摘要:

      Fungi are one of the more common microorganisms in our daily lives, and are closely related to our lives, with both advantages and disadvantages. Its harmful effects are manifested in a number of ways. For example, some fungi can cause crop diseases and reduce yield, accidental consumption of poisonous mushrooms can cause food poisoning and threaten people’s lives, in addition, pathogenic fungi can also cause fungal infections and other diseases that are harmful to people’s health, so it is very important to be able to quickly and accurately detect pathogenic fungi and identify poisonous mushrooms. Loop-mediated isothermal amplification (LAMP) is a nucleic acid amplification technique widely used in recent years, which uses DNA polymerase and specific primers to amplify nucleic acids with high efficiency in a short period of time under constant temperature conditions, compared with traditional molecular biology methods, this method does not require high instrumentation, and is characterized by rapidity, sensitivity and simplicity. At present, LAMP technology has been widely used in the detection of microorganisms such as bacteria, fungi and viruses, and this paper summarised the application of LAMP technology in the detection of fungi to provide a reference for related research.