超高效液相色谱法测定食品中枸杞酸含量

Determination of ascorbic acid 2-glucoside content in food by ultra performance liquid chromatography

中文关键词:  超高效液相色谱法（UPLC）  枸杞酸  食品

英文关键词:ultra performance liquid chromatography  ascorbic acid 2-glucoside  food

中文摘要:

      目的 建立超高效液相色谱法(ultra performance liquid chromatography, UPLC)测定食品中枸杞酸(ascorbic acid 2-glucoside, AA-2βG)含量的分析方法。方法 采用Torus Diol (3.0 mm×100 mm, 1.7 μm)色谱柱, 以乙腈-乙酸铵溶液(85:15, V:V)为流动相进行AA-2βG的分离, 流速为0.4 mL/min, 柱温为40 ℃, 在紫外检测波长260 nm下检测, 外标法定量。结果 AA-2βG在3.01~15.00 μg/mL质量浓度范围内呈现良好的线性关系, 相关系数r2=0.9997, 加标回收率均大于85% (n=9), 相对标准偏差为2.22%, 精密度相对标准偏差为1.33%, 重复性相对标准偏差为1.15%, 本方法的检出限为0.03 μg/g, 定量限为0.09 μg/g。结论 该方法前处理简便, 检测速度快捷、稳定, 灵敏度和准确度高, 可以满足枸杞相关配方产品中AA-2βG的含量测定和质量控制要求, 同时可为行业内对食品中AA-2βG的定性定量分析提供有价值的参考。

英文摘要:

      Objective To establish a method for the determination of ascorbic acid 2-glucoside (AA-2βG) content in food by ultra performance liquid chromatography (UPLC). Methods A Torus Diol (3.0 mm×100 mm, 1.7 μm) column was used, with acetonitrile-ammonium acetate solution (85:15, V:V) as the mobile phase for the separation of AA-2βG, at a flow rate of 0.4 mL/min, column temperature of 40 ℃, and detected at ultraviolet detection wavelength of 260 nm, with external standard quantification. Results The linearity of AA-2βG was good in the range of 3.01–15.00 μg/mL, with a correlation coefficient r2=0.9997. The recovery rates were all greater than 85% (n=9), the relative standard deviation was 2.22%, the relative standard deviation of precision was 1.33%, the relative standard deviation of repeatability was 1.15%, the limit of detection of method was 0.03 μg/g, and the limit of quantification was 0.09 μg/g. Conclusion This method is convenient for pre-treatment, fast in detection speed, stable, and has high sensitivity and accuracy, which can meet the requirements for the determination and quality control of AA-2βG content in related formula products, and can also provide valuable reference for the qualitative and quantitative analysis of AA-2βG in food within the industry.